Reference : Localization of nucleolar chromatin by immunocytochemistry and in situ hybridization ...
Scientific journals : Article
Life sciences : Anatomy (cytology, histology, embryology...) & physiology
http://hdl.handle.net/2268/29285
Localization of nucleolar chromatin by immunocytochemistry and in situ hybridization at the electron microscopic level.
English
Thiry, Marc mailto [Université de Liège - ULg > Département des sciences de la vie > Biologie cellulaire >]
Scheer, U. [> > > >]
Goessens, Guy mailto [Université de Liège - ULg > Services généraux (Faculté des sciences) > Relations académiques et scientifiques (Sciences) >]
1991
Electron Microscopy Reviews
Pergamon Press
4
1
85-110
Yes (verified by ORBi)
International
0892-0354
Elmsford
NY
[en] Animals ; Cell Nucleolus/metabolism/ultrastructure ; Chromatin/metabolism/ultrastructure ; DNA/metabolism ; Histones/metabolism ; Humans ; Immunohistochemistry ; Microscopy, Electron ; Nucleic Acid Hybridization ; RNA, Ribosomal/genetics/metabolism
[en] Nucleoli are the morphological expression of the activity of a defined set of chromosomal segments bearing rRNA genes. The topological distribution and composition of the intranucleolar chromatin as well as the definition of nucleolar structures in which enzymes of the rDNA transcription machinery reside have been investigated in mammalian cells by various immunogold labelling approaches at the ultrastructural level. The precise intranucleolar location of rRNA genes has been further specified by electron microscopic in situ hybridization with a non-autoradiographic procedure. Our results indicate that the fibrillar centers are the sole nucleolar structures where rDNA, core histones, RNA polymerase I and DNA topoisomerase I are located together. Taking into account the potential value and limitations of immunoelectron microscopic techniques, we propose that transcription of the rRNA genes takes place within the confines of the fibrillar centers, probably close to the boundary regions to the surrounding dense fibrillar component.
http://hdl.handle.net/2268/29285

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