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Article (Scientific journals)
Locating transcribed and non-transcribed rDNA spacer sequences within the nucleolus by in situ hybridization and immunoelectron microscopy.
Thiry, Marc; Thiry-Blaise, L.
1991In Nucleic Acids Research, 19 (1), p. 11-5
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Keywords :
Animals; Cell Nucleolus/chemistry/ultrastructure; DNA, Ribosomal/analysis; Mice; Microscopy, Immunoelectron; Nucleic Acid Hybridization; Repetitive Sequences, Nucleic Acid; Transcription, Genetic; Tumor Cells, Cultured
Abstract :
[en] Immunoelectron microscopy and in situ hybridization have been used to investigate the precise location of transcribed and non-transcribed rDNA spacer sequences. Whereas a 5'-external transcribed spacer sequence is predominantly visualized in the fibrillar centers of nucleoli, a non-transcribed spacer sequence is preferentially detected in the interstices, in close contact with the fibrillar centers and which interrupt the surrounding dense fibrillar component. Occasionally these two spacers are also observed in clumps of dense nucleolus-associated chromatin. These observations provide insights into the organization of ribosomal repeats within the nucleolus.
Disciplines :
Anatomy (cytology, histology, embryology...) & physiology
Author, co-author :
Thiry, Marc  ;  Université de Liège - ULiège > Département des sciences de la vie > Biologie cellulaire
Thiry-Blaise, L.
Language :
English
Title :
Locating transcribed and non-transcribed rDNA spacer sequences within the nucleolus by in situ hybridization and immunoelectron microscopy.
Publication date :
1991
Journal title :
Nucleic Acids Research
ISSN :
0305-1048
eISSN :
1362-4962
Publisher :
Oxford University Press, Oxford, United Kingdom
Volume :
19
Issue :
1
Pages :
11-5
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 25 November 2009

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