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Ultrastructural distribution of histones within Ehrlich tumor cell nucleoli: a cytochemical and immunocytochemical study.
Thiry, Marc; Muller, S.
1989In Journal of Histochemistry and Cytochemistry, 37 (6), p. 853-62
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Keywords :
Animals; Antibodies, Monoclonal/immunology; Carcinoma, Ehrlich Tumor/metabolism/pathology/ultrastructure; Cell Nucleolus/metabolism/ultrastructure; DNA/immunology/metabolism; Histocytochemistry; Histones/immunology/metabolism; Immune Sera/immunology; Immunohistochemistry/methods; Mice; Mice, Inbred C57BL; Microscopy, Electron; Phosphotungstic Acid/diagnostic use; Silver Nitrate/diagnostic use; Tumor Cells, Cultured
Abstract :
[en] We investigated the ultrastructural distribution of histones within Ehrlich tumor cell nucleoli by means of three cytochemical methods and by a Lowicryl post-embedding immunogold labeling procedure involving anti-histone (H2B, H3, H4) antisera as well as antibodies to synthetic peptides of histones. With the two technical approaches, labeling was particularly concentrated over the perinucleolar chromatin and over its intranucleolar invaginations which penetrate the nucleolar body and come in close contact with the fibrillar centers. Furthermore, the high-resolution immunocytochemical technique revealed the presence of a small amount of the three histones in the fibrillar centers, preferentially located towards their peripheral regions. In addition, colocalization of DNA at all the histone-positive sites could be visualized after double immunogold staining using a monoclonal anti-DNA antibody and the anti-histone antisera. These results appear to indicate that all the DNA detected within the nucleolus was associated with histones. This finding suggests that the ribosomal DNA, including transcriptionally active genes, is bound to histones.
Disciplines :
Anatomy (cytology, histology, embryology...) & physiology
Author, co-author :
Thiry, Marc  ;  Université de Liège - ULiège > Département des sciences de la vie > Biologie cellulaire
Muller, S.
Language :
English
Title :
Ultrastructural distribution of histones within Ehrlich tumor cell nucleoli: a cytochemical and immunocytochemical study.
Publication date :
1989
Journal title :
Journal of Histochemistry and Cytochemistry
ISSN :
0022-1554
eISSN :
1551-5044
Publisher :
Histochemical Society, New York, United States - New York
Volume :
37
Issue :
6
Pages :
853-62
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 25 November 2009

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