No full text
Article (Scientific journals)
In situ hybridization at the electron microscope level: an improved method for precise localization of ribosomal DNA and RNA.
Thiry, Marc; Thiry-Blaise, L.
1989In European Journal of Cell Biology, 50 (1), p. 235-43
Peer Reviewed verified by ORBi
 

Files


Full Text
No document available.

Send to



Details



Keywords :
Animals; Antibodies; Biotin; Carcinoma, Ehrlich Tumor; Cell Nucleolus/analysis/ultrastructure; DNA, Ribosomal/analysis; DNA, Single-Stranded/immunology; Immunohistochemistry; Mice; Microscopy, Electron; Nucleic Acid Denaturation; Nucleic Acid Hybridization; RNA, Ribosomal/analysis; Tumor Cells, Cultured
Abstract :
[en] In situ hybridization using biotinylated rDNA probes and secondary antibody coupled to gold particles was developed on ultrathin sections of Lowicryl-embedded Ehrlich tumor cells for precise localization of ribosomal RNA (rRNA) and ribosomal DNA (rDNA). For the detection of rDNA, an immunocytochemical approach involving an antibody against single-stranded DNA was used in order to determine the more efficient denaturation procedure. Using this technique, rDNA can be visualized in the fibrillar centers of nucleoli, especially in their peripheral regions at the proximity of both the dense fibrils and the nucleolar interstices as well as within the latter. rDNA was occasionally detected in some clumps of dense nucleolus-associated chromatin. Besides the presence of rRNA in the ribosome-rich cytoplasmic areas and in the dense fibrillar component and the granular component of the nucleolus, rRNA was also found in the fibrillar center areas close to the boundary region to the dense fibrillar component. These results are discussed in the light of the present knowledge on the functional organization of the nucleolus.
Disciplines :
Anatomy (cytology, histology, embryology...) & physiology
Author, co-author :
Thiry, Marc  ;  Université de Liège - ULiège > Département des sciences de la vie > Biologie cellulaire
Thiry-Blaise, L.
Language :
English
Title :
In situ hybridization at the electron microscope level: an improved method for precise localization of ribosomal DNA and RNA.
Publication date :
1989
Journal title :
European Journal of Cell Biology
ISSN :
0171-9335
eISSN :
1618-1298
Publisher :
Wissenschaftliche Verlagsgesellschaft, Stuttgart, Germany
Volume :
50
Issue :
1
Pages :
235-43
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 25 November 2009

Statistics


Number of views
36 (0 by ULiège)
Number of downloads
0 (0 by ULiège)

Scopus citations®
 
127
Scopus citations®
without self-citations
109

Bibliography


Similar publications



Contact ORBi