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Article (Scientific journals)
Anti-bromodeoxyuridine monoclonal antibody: an alternative tool for the identification of replicated DNA at the electron microscope level.
Thiry, Marc; Dombrowicz, D.
1988In Biology of the Cell, 62 (1), p. 99-102
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Keywords :
Animals; Antibodies, Monoclonal/diagnostic use; Bromodeoxyuridine/immunology; Carcinoma, Ehrlich Tumor; DNA/analysis/ultrastructure; DNA Replication; Floxuridine; Immunohistochemistry; Microscopy, Electron; Tumor Cells, Cultured
Abstract :
[en] A new method for identifying the replicated DNA at the electron microscope level is described. Cells were first exposed in vitro to 5-bromodeoxyuridine (BUdR) in conjunction with 5-fluorodeoxyuridine (FUdR) and BUdR incorporated into DNA was then detected on Lowicryl-embedded sections by immunogold technique using a monoclonal anti-BUdR antibody. After using this method, chromatin and chromosomes are strongly labelled.
Disciplines :
Anatomy (cytology, histology, embryology...) & physiology
Author, co-author :
Thiry, Marc  ;  Université de Liège - ULiège > Département des sciences de la vie > Biologie cellulaire
Dombrowicz, D.
Language :
English
Title :
Anti-bromodeoxyuridine monoclonal antibody: an alternative tool for the identification of replicated DNA at the electron microscope level.
Publication date :
1988
Journal title :
Biology of the Cell
ISSN :
0248-4900
eISSN :
1768-322X
Publisher :
Portland Press, Colchester, United Kingdom
Volume :
62
Issue :
1
Pages :
99-102
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 25 November 2009

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