Reference : ERK activation upon hypoxia: involvement in HIF-1 activation.
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Human health sciences : Oncology
http://hdl.handle.net/2268/29243
ERK activation upon hypoxia: involvement in HIF-1 activation.
English
Minet, E. [> > > >]
Arnould, T. [> > > >]
Michel, G. [> > > >]
Roland, I. [ > > ]
Mottet, Denis mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > GIGA-R : Labo de recherche sur les métastases >]
Raes, M. [> > > >]
Remacle, J. [ > > ]
Michiels, C. [> > > >]
2000
FEBS Letters
Elsevier Science
468
1
53-8
Yes (verified by ORBi)
International
0014-5793
Amsterdam
The Netherlands
[en] Blotting, Western ; Cell Hypoxia/physiology ; Cell Line ; Cell Nucleus/enzymology ; DNA-Binding Proteins/metabolism ; Endothelium, Vascular/cytology/enzymology ; Enzyme Activation/drug effects ; Enzyme Inhibitors/pharmacology ; Flavonoids/pharmacology ; Humans ; Hypoxia-Inducible Factor 1 ; Hypoxia-Inducible Factor 1, alpha Subunit ; Microcirculation/cytology/enzymology ; Mitogen-Activated Protein Kinase 1/antagonists & inhibitors/metabolism ; Mitogen-Activated Protein Kinase 3 ; Mitogen-Activated Protein Kinases/antagonists & inhibitors/metabolism ; Nuclear Proteins/metabolism ; Phosphorylation ; Precipitin Tests ; Protein Structure, Tertiary ; Transcription Factors/metabolism ; Tyrosine/metabolism
[en] Hypoxia-inducible factor-1 (HIF-1) is a transcription factor activated by hypoxia. The HIF-1 activation transduction pathway is poorly understood. In this report, we investigated the activation of extracellular regulated kinases (ERK) in hypoxia and their involvement in HIF-1 activation. We demonstrated that in human microvascular endothelial cells-1 (HMEC-1), ERK kinases are activated during hypoxia. Using dominant negative mutants, we showed that ERK1 is needed for hypoxia-induced HIF-1 transactivation activity. Moreover, using a kinase assay and Western blot experiments, we showed that HIF-1alpha is phosphorylated in hypoxia by an ERK-dependent pathway. These results evidence the role of mitogen-activated protein kinase in the transcriptional response to hypoxia.
Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS ; Fonds pour la Recherche dans l'Industrie et l'Agriculture
http://hdl.handle.net/2268/29243

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Restricted access
FEBS Letters 2000.pdfPublisher postprint344.76 kBRequest copy

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.