ORBi: Minet E. - ERK activation upon hypoxia: involvement in HIF-1 activation.

Reference : ERK activation upon hypoxia: involvement in HIF-1 activation.


	Document type :	Scientific journals : Article
	Discipline(s) :	Human health sciences : Oncology Life sciences : Biochemistry, biophysics & molecular biology
	To cite this reference:	http://hdl.handle.net/2268/29243

Title :	ERK activation upon hypoxia: involvement in HIF-1 activation.
Language :	English
Author, co-author :	Minet, E. [> > > >]
	Arnould, T. [> > > >]
	Michel, G. [> > > >]
	Roland, I. [ > > ]
	Mottet, Denis [Université de Liège - ULg > Département des sciences biomédicales et précliniques > GIGA-R : Labo de recherche sur les métastases >]
	Raes, M. [> > > >]
	Remacle, J. [ > > ]
	Michiels, C. [> > > >]
Publication date :	2000
Journal title :	FEBS Letters
Publisher :	Elsevier Science
Volume :	468
Issue/season :	1
Pages :	53-8
Audience :	International
ISSN :	0014-5793
City :	Amsterdam
Country :	The Netherlands
Keywords :	[en] Blotting, Western ; Cell Hypoxia/physiology ; Cell Line ; Cell Nucleus/enzymology ; DNA-Binding Proteins/metabolism ; Endothelium, Vascular/cytology/enzymology ; Enzyme Activation/drug effects ; Enzyme Inhibitors/pharmacology ; Flavonoids/pharmacology ; Humans ; Hypoxia-Inducible Factor 1 ; Hypoxia-Inducible Factor 1, alpha Subunit ; Microcirculation/cytology/enzymology ; Mitogen-Activated Protein Kinase 1/antagonists & inhibitors/metabolism ; Mitogen-Activated Protein Kinase 3 ; Mitogen-Activated Protein Kinases/antagonists & inhibitors/metabolism ; Nuclear Proteins/metabolism ; Phosphorylation ; Precipitin Tests ; Protein Structure, Tertiary ; Transcription Factors/metabolism ; Tyrosine/metabolism
Abstract :	[en] Hypoxia-inducible factor-1 (HIF-1) is a transcription factor activated by hypoxia. The HIF-1 activation transduction pathway is poorly understood. In this report, we investigated the activation of extracellular regulated kinases (ERK) in hypoxia and their involvement in HIF-1 activation. We demonstrated that in human microvascular endothelial cells-1 (HMEC-1), ERK kinases are activated during hypoxia. Using dominant negative mutants, we showed that ERK1 is needed for hypoxia-induced HIF-1 transactivation activity. Moreover, using a kinase assay and Western blot experiments, we showed that HIF-1alpha is phosphorylated in hypoxia by an ERK-dependent pathway. These results evidence the role of mitogen-activated protein kinase in the transcriptional response to hypoxia.
Funders :	Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS ; Fonds pour la Recherche dans l'Industrie et l'Agriculture
Permalink :	http://hdl.handle.net/2268/29243

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