Article (Scientific journals)
ERK activation upon hypoxia: involvement in HIF-1 activation.
Minet, E.; Arnould, T.; Michel, G. et al.
2000In FEBS Letters, 468 (1), p. 53-8
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Keywords :
Blotting, Western; Cell Hypoxia/physiology; Cell Line; Cell Nucleus/enzymology; DNA-Binding Proteins/metabolism; Endothelium, Vascular/cytology/enzymology; Enzyme Activation/drug effects; Enzyme Inhibitors/pharmacology; Flavonoids/pharmacology; Humans; Hypoxia-Inducible Factor 1; Hypoxia-Inducible Factor 1, alpha Subunit; Microcirculation/cytology/enzymology; Mitogen-Activated Protein Kinase 1/antagonists & inhibitors/metabolism; Mitogen-Activated Protein Kinase 3; Mitogen-Activated Protein Kinases/antagonists & inhibitors/metabolism; Nuclear Proteins/metabolism; Phosphorylation; Precipitin Tests; Protein Structure, Tertiary; Transcription Factors/metabolism; Tyrosine/metabolism
Abstract :
[en] Hypoxia-inducible factor-1 (HIF-1) is a transcription factor activated by hypoxia. The HIF-1 activation transduction pathway is poorly understood. In this report, we investigated the activation of extracellular regulated kinases (ERK) in hypoxia and their involvement in HIF-1 activation. We demonstrated that in human microvascular endothelial cells-1 (HMEC-1), ERK kinases are activated during hypoxia. Using dominant negative mutants, we showed that ERK1 is needed for hypoxia-induced HIF-1 transactivation activity. Moreover, using a kinase assay and Western blot experiments, we showed that HIF-1alpha is phosphorylated in hypoxia by an ERK-dependent pathway. These results evidence the role of mitogen-activated protein kinase in the transcriptional response to hypoxia.
Disciplines :
Oncology
Biochemistry, biophysics & molecular biology
Author, co-author :
Minet, E.
Arnould, T.
Michel, G.
Roland, I.
Mottet, Denis ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > GIGA-R : Labo de recherche sur les métastases
Raes, M.
Remacle, J.
Michiels, C.
Language :
English
Title :
ERK activation upon hypoxia: involvement in HIF-1 activation.
Publication date :
2000
Journal title :
FEBS Letters
ISSN :
0014-5793
eISSN :
1873-3468
Publisher :
Elsevier Science, Amsterdam, Netherlands
Volume :
468
Issue :
1
Pages :
53-8
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
IRSIA - Institut pour l'Encouragement de la Recherche Scientifique dans l'Industrie et l'Agriculture [BE]
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