Article (Scientific journals)
Madin Darby Bovine Kidney Cell Synchronization by Lovastatin: Application to Bovine Herpesvirus-1 Gene Expression
Vanderplasschen, Alain; Hanon, E.; Benarafa, C. et al.
1994In Veterinary Research, 25 (6), p. 555-67
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Keywords :
MDBK cells; cell-cycle synchronization; lovastatin; bovine herpesvirus-1
Abstract :
[en] The number of investigations involving cell proliferation has increased rapidly in the last years. One of the major difficulties in studying cell-cycle-related events is obtaining highly synchronous cell populations without metabolic imbalance. This study demonstrates that the Madin Darby bovine kidney (MDBK) cells, a commonly used cell line in veterinary research, can be effectively synchronized using lovastatin (Lov), a drug used to treat hypercholesteremia in humans. This was demonstrated by the following results: (i) Lov inhibits cell proliferation in a dose-dependent manner; (ii) Lov synchronizes MDBK cells mainly in the G1 and secondarily in the G2+M cell-cycle phases; (iii) the cytostatic effect of Lov can be specifically inhibited by addition of mevalonate (Mev) (Lov inhibits the synthesis of Mev); (iv) removal of Lov from G1-arrested cultures, followed by addition of Mev, resulted in the synchronous recovery of DNA synthesis; and (v) 5-bromo2'-deoxyuridine incorporation experiments revealed that MDBK cells synchronization by Lov can be followed for at least 3 cycles after removal of Lov and addition of Mev. Furthermore, as an application of investigations based on the availability of synchronized MDBK, we showed that bovine herpesvirus-1 gene expression is independent on the cell cycle.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Vanderplasschen, Alain ;  Université de Liège - ULiège > Faculté de médecine vétérinaire > Virologie et vaccinologie
Hanon, E.;  Université de Liège - ULiège > Faculté de médecine vétérinaire > Virologie et vaccinologie
Benarafa, C.;  Université de Liège - ULiège > Faculté de médecine vétérinaire > Virologie et vaccinologie
Greimers, Roland ;  Université de Liège - ULiège > Faculté de médecine vétérinaire > Virologie et vaccinologie
Op De Beeck, A.;  Université de Liège - ULiège > Faculté de médecine vétérinaire > Virologie et vaccinologie
Loncar, M.;  Université de Liège - ULiège > Faculté de médecine vétérinaire > Virologie et vaccinologie
Pastoret, Paul-Pierre ;  Université de Liège - ULiège > Faculté de médecine vétérinaire > Virologie et vaccinologie
Language :
English
Title :
Madin Darby Bovine Kidney Cell Synchronization by Lovastatin: Application to Bovine Herpesvirus-1 Gene Expression
Publication date :
1994
Journal title :
Veterinary Research
ISSN :
0928-4249
eISSN :
1297-9716
Publisher :
BioMed Central, United Kingdom
Volume :
25
Issue :
6
Pages :
555-67
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 24 November 2009

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