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Study of RNA distribution in the nucleolar components of Ehrlich cell using RNase-gold method.
Thiry, Marc
1988In Histochemistry, 89 (3), p. 231-6
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Keywords :
Animals; Carcinoma, Ehrlich Tumor/metabolism/ultrastructure; Cell Nucleolus/metabolism/ultrastructure; Cells, Cultured; Gold; Histocytochemistry; Mice; Microscopy, Electron; RNA Precursors/metabolism; RNA, Neoplasm/metabolism; Ribonucleases
Abstract :
[en] The RNA distribution in Ehrlich tumour cell nucleoli has been investigated using RNase-gold method. This technique has been applied to sections of cells prepared under various fixation and embedding conditions. As expected, the specificity and intensity of labelling by gold particles have varied according to the experimental conditions used. Interestingly, however, it has been noted that the localization of gold particles does also vary and in particular within the fibrillar centre. This observation underlines the interest of assaying the RNase-gold complex under various conditions. The gold particles were particularly concentrated over the granular component and to a lesser extent, in the dense fibrillar component. In the latter constituent, it has been noted that the gold markers were preferentially localized at the edge of the dense fibrils. Surprisingly, a few gold particles have also been detected in the fibrillar centres. The weak labelling has persisted even after pepsin or DNase extraction but has completely disappeared after RNase extraction. Further, an inhibition of rRNA synthesis by a treatment with actinomycin D has not produced a significant decrease of the number of gold particles present in the fibrillar centre. These results suggest that fibrillar centres contain a small amount of RNA which would not correspond to pre-rRNA.
Disciplines :
Anatomy (cytology, histology, embryology...) & physiology
Author, co-author :
Thiry, Marc  ;  Université de Liège - ULiège > Département des sciences de la vie > Biologie cellulaire
Language :
English
Title :
Study of RNA distribution in the nucleolar components of Ehrlich cell using RNase-gold method.
Publication date :
1988
Journal title :
Histochemistry
ISSN :
0301-5564
Publisher :
Springer Verlag, Berlin, Germany
Volume :
89
Issue :
3
Pages :
231-6
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 24 November 2009

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