Article (Scientific journals)
Downregulation of ICAM-1 and VCAM-1 expression in endothelial cells treated by photodynamic therapy
Volanti, Cédric; Gloire, Geoffrey; Vanderplasschen, Alain et al.
2004In Oncogene, 23 (53), p. 8649-8658
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Keywords :
NF-kappa B; pyropheophorbide; photodynamic therapy; endothelial cells; oxidative stress; adhesion molecules
Abstract :
[en] Photodynamic therapy (PDT) is a treatment for cancer and several noncancerous proliferating cell diseases that depends on the uptake of a photosensitizing compound followed by selective irradiation with visible light. In the presence of oxygen, irradiation leads to the production of reactive oxygen species (ROS). A large production of ROS induces the death of cancer cells by apoptosis or necrosis. A small ROS production can activate various cellular pathways. Here, we show that PDT by pyropheophorbide-a methyl ester (PPME) induces the activation of nuclear factor kappa B (NF-kappaB) in HMEC-1 cells. NF-kappaB is active since it binds to the NF-kappaB sites of both ICAM-1 and vascular cell adhesion molecule-1 (VCAM-1) promoters and induces the transcription of several NF-kappaB target genes such as those of IL-6, ICAM-1, VCAM-1. In contrast, expression of ICAM-1 and VCAM-1 at the protein level was not observed, although we measured an IL-6 secretion. Using specific chemical inhibitors, we showed that the lack of ICAM-1 and VCAM-1 expression is the consequence of their degradation by lysosomal proteases. The proteasome and calpain pathways were not involved. All these observations were consistent with the fact that no adhesion of granulocytes was observed in these conditions.
Disciplines :
Oncology
Biochemistry, biophysics & molecular biology
Author, co-author :
Volanti, Cédric ;  Université de Liège - ULiège > Département de morphologie et pathologie > Embryologie
Gloire, Geoffrey ;  Université de Liège - ULiège > Virologie - Immunologie
Vanderplasschen, Alain ;  Université de Liège - ULiège > Immunologie et vaccinologie
Jacobs, Nathalie  ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques
Habraken, Yvette ;  Université de Liège - ULiège > Virologie - Immunologie
Piette, Jacques ;  Université de Liège - ULiège > Département des sciences de la vie > Virologie - Immunologie
Language :
English
Title :
Downregulation of ICAM-1 and VCAM-1 expression in endothelial cells treated by photodynamic therapy
Publication date :
2004
Journal title :
Oncogene
ISSN :
0950-9232
eISSN :
1476-5594
Publisher :
Nature Publishing Group, London, United Kingdom
Volume :
23
Issue :
53
Pages :
8649-8658
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 24 November 2009

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