Reference : The core 2 beta-1,6-N-acetylglucosaminyltransferase-M encoded by bovine herpesvirus 4 is...
Scientific journals : Article
Life sciences : Veterinary medicine & animal health
http://hdl.handle.net/2268/29147
The core 2 beta-1,6-N-acetylglucosaminyltransferase-M encoded by bovine herpesvirus 4 is not essential for virus replication despite contributing to post-translational modifications of structural proteins.
English
Markine-Goriaynoff, Nicolas [> > > >]
Gillet, Laurent mailto [Université de Liège - ULg > > Immunologie et vaccinologie >]
Karlsen, Odd A [> > > >]
Haarr, Lars [> > > >]
Minner, Frederic [> > > >]
Pastoret, Paul-Pierre mailto [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Département des maladies infectieuses et parasitaires >]
Fukuda, Minoru [> > > >]
Vanderplasschen, Alain mailto [Université de Liège - ULg > > Immunologie et vaccinologie >]
2004
Journal of General Virology (The)
Society for General Microbiology
85
Pt 2
355-67
Yes (verified by ORBi)
International
0022-1317
1465-2099
London
United Kingdom
[en] Enzyme Induction ; Gene Deletion ; Gene Expression ; Herpesvirus 4, Bovine/genetics/metabolism ; N-Acetylglucosaminyltransferases/biosynthesis/genetics/metabolism ; Protein Processing, Post-Translational ; Species Specificity ; Viral Structural Proteins/metabolism ; Virus Replication
[en] The Bo17 gene of bovine herpesvirus 4 (BoHV-4) is the only virus gene known to date that encodes a homologue of the cellular core 2 beta-1,6-N-acetylglucosaminyltransferase-mucine type (C2GnT-M). Recently, our phylogenetic study revealed that the Bo17 gene has been acquired from an ancestor of the African buffalo around 1.5 million years ago. Despite this recent origin, the Bo17 sequence has spread to fixation in the virus population possibly by natural selection. Supporting the latter hypothesis, it has been shown by our group for the V. test strain that Bo17 is expressed during BoHV-4 replication in vitro, and that Bo17 expression product (pBo17) has all three enzymic activities exhibited by cellular C2GnT-M, i.e. core 2, core 4 and I branching activities. In the present study, firstly it was investigated whether encoding a functional C2GnT-M is a general property of BoHV-4 strains. Analysis of nine representative strains of the BoHV-4 species revealed that all of them express the Bo17 gene and the associated core 2 branching activity during virus replication in vitro. Secondly, in order to investigate the roles of Bo17, its kinetic class of expression was analysed and a deleted recombinant strain was produced. These experiments revealed that Bo17 is expressed as an early gene which is not essential for virus replication in vitro. However, comparison of the structural proteins, produced by the wild-type, the revertant and the deleted viruses, by 2D gels demonstrated that pBo17 contributes to the post-translational modifications of structural proteins. Possible roles of Bo17 in vivo are discussed.
http://hdl.handle.net/2268/29147

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