|Reference : Behaviour of nucleolus during mitosis. A comparative ultrastructural study of various ca...|
|Scientific journals : Article|
|Life sciences : Anatomy (cytology, histology, embryology...) & physiology|
|Behaviour of nucleolus during mitosis. A comparative ultrastructural study of various cancerous cell lines using the Ag-NOR staining procedure.|
|Ploton, D. [> > > >]|
|Thiry, Marc [Université de Liège - ULg > Département des sciences de la vie > Biologie cellulaire >]|
|Menager, M. [> > > >]|
|Lepoint, A. [> > > >]|
|Adnet, J. J. [> > > >]|
|Goessens, Guy [Université de Liège - ULg > Services généraux (Faculté des sciences) > Relations académiques et scientifiques (Sciences) >]|
|[en] Anaphase ; Animals ; Carcinoma, Ehrlich Tumor/pathology/ultrastructure ; Cell Line ; Cell Nucleolus/ultrastructure ; Chromatin/ultrastructure ; Humans ; Leukemia L1210/pathology ; Metaphase ; Mice ; Microscopy, Electron ; Mitosis ; Nucleolus Organizer Region/ultrastructure ; Staining and Labeling|
|[en] The aim of the present work was to study the distribution and the behaviour of the silver-staining nucleolar organizer region (Ag-NOR) proteins at the ultrastructural level during interphase and mitosis in five human and murine cancerous cell lines each characterized by a typical nucleolar morphology. During interphase the Ag-NOR proteins are restricted to the fibrillar centres (F.C.) and/or to the dense fibrillar component (D.F.C.). During prophase the silver-staining components come into close contact with some chromosomes and are arranged with a typical polarity: chromosome, F.C. and D.F.C. Then F.C. and D.F.C. together form roundish silver-stained structures and integrate in part within indentations at the periphery of the metaphase chromosomes. During anaphase and telophase large and small spherical silver-staining structures may be seen. They correspond respectively to the metaphase NORs and to numerous structures which appear de novo within ribonucleoprotein (RNP) material localized between the chromosomes. During late telophase the number of the small silver-staining structures decreases whereas the size of the larger ones increases. Then the interphase nucleoli recover their typical shape. These results suggest that when rRNA synthesis is impaired during mitosis the inactive NORs assume a structure and a localization which are not typical of the cell line. In contrast the F.C. and D.F.C. are probably two aspects of the NORs whose typical distribution, relative to the other nucleolar components, gives the interphasic nucleolus its characteristic morphology.|
There is no file associated with this reference.
All documents in ORBi are protected by a user license.