Bovine herpesvirus 4 induces apoptosis of human carcinoma cell lines in vitro and in vivo

[en] The idea of using oncolytic viruses for the treatment of cancers was proposed a century ago. During the last two decades, viruses able to replicate specifically in cancer cells and to induce their lysis were identified and were genetically modified to improve their viro-oncolytic properties. More recently, a new approach consisting of inducing selective apoptosis in cancer cells through viral infection has been proposed; this approach has been called viro-oncoapoptosis. In the present study, we report the property of bovine herpesvirus-4 (BoHV-4) to induce, in vitro and in vivo, apoptosis of some human carcinomas. This conclusion relies on the following observations: (a) In vitro, BoHV-4 infection induced apoptosis of A549 and OVCAR carcinoma cell lines in a time- and dose-dependent manner. (b) Apoptosis was induced by the expression of an immediate-early or an early BoHV-4 gene, but did not require viral replication. (c) Cell treatment with caspase inhibitors showed that apoptosis induced by BoHV-4 relied mainly on caspase-10 activation. (d) Infection of cocultures of A549 or OVCAR cells mixed with human 293 cells (in which BoHV-4 does not induce apoptosis) showed that BoHV-4 specifically eradicated A549 or OVCAR cancer cells from the cocultures. (e) Finally, in vivo experiments done with nude mice showed that BoHV-4 intratumoral injections reduced drastically the growth of preestablished A549 xenografts. Taken together, these results suggest that BoHV-4 may have potential as a viro-oncoapoptotic agent for the treatment of some human carcinomas. Moreover, further identification of BoHV-4 proapoptotic gene(s) and the cellular pathways targeted by this or these gene(s) could lead to the design of new cancer therapeutic strategies.

Disciplines :

Oncology

Author, co-author :

Gillet, Laurent ; Université de Liège - ULiège > Immunologie et vaccinologie

Dewals, Benjamin G ; Université de Liège - ULiège > Immunologie et vaccinologie

Farnir, Frédéric ; Université de Liège - ULiège > Département de productions animales > Biostatistique, économie, sélection animale

de Leval, Laurence ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques

Vanderplasschen, Alain ; Université de Liège - ULiège > Immunologie et vaccinologie

Language :

English

Title :

Bovine herpesvirus 4 induces apoptosis of human carcinoma cell lines in vitro and in vivo

Publication date :

2005

Journal title :

Cancer Research

ISSN :

0008-5472

eISSN :

1538-7445

Publisher :

Amer Assoc Cancer Research, Philadelphia, United States - Pennsylvania

Volume :

Issue :

Pages :

9463-9472

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 24 November 2009

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