ORBi: Hamilton Christopher - Optimisation of culture conditions for biological hydrogen production by Citrobacter freundii CWBI952 in batch, sequenced-batch and semicontinuous operating mode

Reference : Optimisation of culture conditions for biological hydrogen production by Citrobacter fre...


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Microbiology Life sciences : Biotechnology
	To cite this reference:	http://hdl.handle.net/2268/29130

Title :	Optimisation of culture conditions for biological hydrogen production by Citrobacter freundii CWBI952 in batch, sequenced-batch and semicontinuous operating mode
Language :	English
Author, co-author :	Hamilton, Christopher [Université de Liège - ULg > > Centre Wallon de biologie industrielle >]
	Hiligsmann, Serge [Université de Liège - ULg > Département des sciences de la vie > Biochimie et microbiologie industrielles >]
	Beckers, Laurent [Université de Liège - ULg > Département des sciences de la vie > Biochimie et microbiologie industrielles >]
	Masset, Julien [Université de Liège - ULg > Département des sciences de la vie > Biochimie et microbiologie industrielles >]
	Wilmotte, Annick [Université de Liège - ULg > Département des sciences de la vie > Physiologie et génétique bactériennes >]
	Thonart, Philippe [Université de Liège - ULg > > Gembloux Agro-Bio Tech >]
Publication date :	2010
Journal title :	International Journal of Hydrogen Energy
Publisher :	Pergamon Press - An Imprint of Elsevier Science
Volume :	35
Pages :	1089-1098
Audience :	International
ISSN :	0360-3199
Keywords :	[en] Biohydrogen ; Citrobacter freundii ; pH ; ammonia ; iron ; dilution
Abstract :	[en] Investigations were carried out to determine the effect of the pH, the nitrogen source, iron and the dilution rate (h 1) on fermentative hydrogen production from glucose by the newly isolated strain Citrobacter freundii CWBI952. The hydrogen production rate (HPR), hydrogen yield, biomass and soluble metabolites were monitored at 30 C in 100 mL serum bottles and in a 2.3 L bioreactor operated in batch, sequenced-batch and semicontinuous mode. The results indicate that hydrogen production activity, formate biosynthesis and glucose intake rates are very sensitive to the culture pH, and that additional formate bioconversion and production of hydrogen with lower biomass yields can be obtained at pH 5.9. In a further series of cultures casein peptone was replaced by (NH4)2SO4, a low cost alternative nitrogen source. The ammonia-based substitute was found to be suitable for H2 production when a concentration of 0.045 g/L FeSO4 was provided. Optimal overall performances (ca. an HPR of 33.2 mL H2/L h and a yield of 0:83 molH2 =molglucose) were obtained in the semicontinuous culture applying the previously optimized parameters for pH, nitrogen, and iron with a dilution rate of 0.012 h 1 and degassing of biogas by N2 at a 28 mL/min flow rate.
Research units :	Centre Wallon de Biologie Industrielle ; Centre d'Ingénierie des Protéines - CIP
Funders :	Action de Recherches concertées ARC 07/12 04- ULg- Communauté française ; Fonds pour la formation à la Recherche dans l'Industrie et dans l'Agriculture (Communauté française de Belgique) - FRIA ; Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS ; Région wallonne : Direction générale des Technologies, de la Recherche et de l'Energie - DGTRE
Name of the research project :	Micro-H2 : Production microbiologique d’hydrogène : Etude des processus microalgal et bacterien
Target :	Researchers ; Professionals ; Students ; General public
Permalink :	http://hdl.handle.net/2268/29130
DOI :	10.1016/j.ijhydene.2009.10.073
Other URL :	http://cwbi.fsagx.ac.be/

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