Reference : Optimisation of culture conditions for biological hydrogen production by Citrobacter fre...
Scientific journals : Article
Life sciences : Biotechnology
Life sciences : Microbiology
http://hdl.handle.net/2268/29130
Optimisation of culture conditions for biological hydrogen production by Citrobacter freundii CWBI952 in batch, sequenced-batch and semicontinuous operating mode
English
Hamilton, Christopher [Université de Liège - ULg > > Centre Wallon de biologie industrielle >]
Hiligsmann, Serge mailto [Université de Liège - ULg > Département des sciences de la vie > Biochimie et microbiologie industrielles >]
Beckers, Laurent mailto [Université de Liège - ULg > Département des sciences de la vie > Biochimie et microbiologie industrielles >]
Masset, Julien mailto [Université de Liège - ULg > Département des sciences de la vie > Biochimie et microbiologie industrielles >]
Wilmotte, Annick mailto [Université de Liège - ULg > Département des sciences de la vie > Physiologie et génétique bactériennes >]
Thonart, Philippe mailto [Université de Liège - ULg > > Gembloux Agro-Bio Tech >]
2010
International Journal of Hydrogen Energy
Pergamon Press - An Imprint of Elsevier Science
35
1089-1098
Yes (verified by ORBi)
International
0360-3199
[en] Biohydrogen ; Citrobacter freundii ; pH ; ammonia ; iron ; dilution
[en] Investigations were carried out to determine the effect of the pH, the nitrogen source, iron
and the dilution rate (h 1) on fermentative hydrogen production from glucose by the newly
isolated strain Citrobacter freundii CWBI952. The hydrogen production rate (HPR), hydrogen
yield, biomass and soluble metabolites were monitored at 30 C in 100 mL serum bottles
and in a 2.3 L bioreactor operated in batch, sequenced-batch and semicontinuous mode.
The results indicate that hydrogen production activity, formate biosynthesis and glucose
intake rates are very sensitive to the culture pH, and that additional formate bioconversion
and production of hydrogen with lower biomass yields can be obtained at pH 5.9. In
a further series of cultures casein peptone was replaced by (NH4)2SO4, a low cost alternative
nitrogen source. The ammonia-based substitute was found to be suitable for H2
production when a concentration of 0.045 g/L FeSO4 was provided. Optimal overall
performances (ca. an HPR of 33.2 mL H2/L h and a yield of 0:83 molH2 =molglucose) were
obtained in the semicontinuous culture applying the previously optimized parameters for
pH, nitrogen, and iron with a dilution rate of 0.012 h 1 and degassing of biogas by N2 at
a 28 mL/min flow rate.
Centre Wallon de Biologie Industrielle ; Centre d'Ingénierie des Protéines - CIP
Action de Recherches concertées ARC 07/12 04- ULg- Communauté française ; Fonds pour la formation à la Recherche dans l'Industrie et dans l'Agriculture (Communauté française de Belgique) - FRIA ; Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS ; Région wallonne : Direction générale des Technologies, de la Recherche et de l'Energie - DGTRE
Micro-H2 : Production microbiologique d’hydrogène : Etude des processus microalgal et bacterien
Researchers ; Professionals ; Students ; General public
http://hdl.handle.net/2268/29130
10.1016/j.ijhydene.2009.10.073
http://cwbi.fsagx.ac.be/

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