Reference : Activation mechanism of recombinant Der p 3 allergen zymogen - Contribution of cysteine ...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/28836
Activation mechanism of recombinant Der p 3 allergen zymogen - Contribution of cysteine protease Der p 1 and effect of propeptide glycosylation
English
Dumez, Marie-Eve mailto [Université de Liège - ULg > Département des sciences de la vie > Macromolécules biologiques, Centre d'Ingénierie des Protéines > >]
Teller, Nathalie [> > > >]
Mercier, Frédéric mailto [Université de Liège - ULg > > Centre de recherches du cyclotron >]
Tanaka, Tetsuya [> > > >]
Vandenberghe, Isabel [> > > >]
Vandenbranden, Michel [> > > >]
Devreese, Bart [> > > >]
Luxen, André mailto [Université de Liège - ULg > Département de chimie (sciences) > Chimie organique de synthèse - Centre de recherches du cyclotron >]
Frère, Jean-Marie mailto [Université de Liège - ULg > > Centre d'ingénierie des protéines >]
Matagne, André mailto [Université de Liège - ULg > Département des sciences de la vie > Enzymologie et repliement des protéines, Centre d'Ingénierie des Protéines > >]
Jacquet, Alain [> > > >]
Galleni, Moreno mailto [Université de Liège - ULg > Département des sciences de la vie > Macromolécules biologiques, Centre d'Ingénierie des Protéines > >]
Chevigne, Andy [Université de Liège > Département des Sciences de la Vie > Macromolécules biologiques, Centre d'Ingénierie des Protéines > >]
2008
Journal of Biological Chemistry
American Society for Biochemistry and Molecular Biology
283
45
30606-30617
Yes (verified by ORBi)
International
0021-9258
1083-351X
Baltimore
MD
[en] The trypsin-like protease Der p 3, a major allergen of the house dust mite Dermatophagoides pteronyssinus, is synthesized as a zymogen, termed proDer p 3. No recombinant source of Der p 3 has been described yet, and the zymogen maturation mechanism remains to be elucidated. The Der p 3 zymogen was produced in Pichia pastoris. We demonstrated that the recombinant zymogen is glycosylated at the level of its propeptide. We showed that the activation mechanism of proDer p 3 is intermolecular and is mediated by the house dust mite cysteine protease Der p 1. The primary structure of the proDer p 3 propeptide is associated with a unique zymogen activation mechanism, which is different from those described for the trypsin-like family and relies on the house dust mite papain-like protease Der p 1. This is the first report of a recombinant source of Der p 3, with the same enzymatic activity as the natural enzyme and trypsin. Glycosylation of the propeptide was found to decrease the rate of maturation. Finally, we showed that recombinant Der p 3 is inhibited by the free modified prosequence TP1R.
http://hdl.handle.net/2268/28836
10.1074/jbc.M803041200

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