Reference : Immuno-quantitative polymerase chain reaction for detection and quantitation of prion...
Scientific journals : Article
Human health sciences : Immunology & infectious disease
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/2876
Immuno-quantitative polymerase chain reaction for detection and quantitation of prion protein
English
[fr] Immuno PCR quantitative pour la détection et la quantification de la protéine prion
Gofflot, Stéphanie mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie Humaine > >]
Elmoualij, Benaïssa mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie humaine >]
Zorzi, Danièle mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques >]
Melen-Lamalle, Laurence mailto [Université de Liège - ULg > Département des sciences cliniques > Labo de biologie des tumeurs et du développement >]
Roels, Stéphane [> > > >]
Quatpers, Dominique [> > > >]
Grassi, Jacques [> > > >]
Vanopdenbosch, Emmanuelle [> > > >]
Heinen, Ernst mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie humaine >]
Zorzi, Willy mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie humaine >]
Aug-2004
Journal of Immunoassay & Immunochemistry
Marcel Dekker Inc
25
3
241-258
Yes (verified by ORBi)
International
1532-1819
New York
[en] immuno-quantitative PCR ; prion ; ELISA
[en] Immuno-polymerase chain reaction (PCR) is an extremely sensitive detection method, combining the specificity of antibody detection and the sensitivity of PCR. We have developed an immuno-quantitative PCR (iqPCR), exploiting real-time PCR technology, in order to improve this immuno-detection method and make it quantitative. To illustrate the advantages of iqPCR, we have compared it with a conventional enzyme linked immuno sorbent assay (ELISA) technique in experiments aimed at detecting the cellular and the resistant form of prion protein in bovine brain extract. The iqPCR technique proved to be more sensitive than ELISA, so it could be a technique of choice for the diagnosis of infected animals both at an ante mortem and post-mortem stage.
Centre de Recherche sur les Protéines Prions
Région wallonne : Direction générale des Technologies, de la Recherche et de l'Energie - DGTRE
http://hdl.handle.net/2268/2876

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