ORBi: Gofflot Stéphanie - Immuno-quantitative polymerase chain reaction for detection and quantitation of prion protein

Reference : Immuno-quantitative polymerase chain reaction for detection and quantitation of prion pr...


	Document type :	Scientific journals : Article
	Discipline(s) :	Human health sciences : Immunology & infectious disease Life sciences : Biochemistry, biophysics & molecular biology
	To cite this reference:	http://hdl.handle.net/2268/2876

Title :	Immuno-quantitative polymerase chain reaction for detection and quantitation of prion protein
Language :	English
Alternative title :	[fr] Immuno PCR quantitative pour la détection et la quantification de la protéine prion
Author, co-author :	Gofflot, Stéphanie [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie Humaine > >]
	Elmoualij, Benaïssa [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie humaine >]
	Zorzi, Danièle [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques >]
	Melen-Lamalle, Laurence [Université de Liège - ULg > Département des sciences cliniques > Labo de biologie des tumeurs et du développement >]
	Roels, Stéphane [> > > >]
	Quatpers, Dominique [> > > >]
	Grassi, Jacques [> > > >]
	Vanopdenbosch, Emmanuelle [> > > >]
	Heinen, Ernst [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie humaine >]
	Zorzi, Willy [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie humaine >]
Publication date :	Aug-2004
Journal title :	Journal of Immunoassay & Immunochemistry
Publisher :	Marcel Dekker Inc
Volume :	25
Issue/season :	3
Pages :	241-258
Audience :	International
ISSN :	1532-1819
City :	New York
Keywords :	[en] immuno-quantitative PCR ; prion ; ELISA
Abstract :	[en] Immuno-polymerase chain reaction (PCR) is an extremely sensitive detection method, combining the specificity of antibody detection and the sensitivity of PCR. We have developed an immuno-quantitative PCR (iqPCR), exploiting real-time PCR technology, in order to improve this immuno-detection method and make it quantitative. To illustrate the advantages of iqPCR, we have compared it with a conventional enzyme linked immuno sorbent assay (ELISA) technique in experiments aimed at detecting the cellular and the resistant form of prion protein in bovine brain extract. The iqPCR technique proved to be more sensitive than ELISA, so it could be a technique of choice for the diagnosis of infected animals both at an ante mortem and post-mortem stage.
Research units :	Centre de Recherche sur les Protéines Prions
Funders :	Région wallonne : Direction générale des Technologies, de la Recherche et de l'Energie - DGTRE
Permalink :	http://hdl.handle.net/2268/2876

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