Reference : Distribution of Aromatase in the Brain of the Japanese Quail, Ring Dove, and Zebra Finch...
Scientific journals : Article
Life sciences : Zoology
Social & behavioral sciences, psychology : Neurosciences & behavior
Human health sciences : Endocrinology, metabolism & nutrition
Distribution of Aromatase in the Brain of the Japanese Quail, Ring Dove, and Zebra Finch: An Immunocytochemical Study
Balthazart, Jacques mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Biologie de la différenciation sexuelle du cerveau >]
Foidart, Agnès mailto [Université de Liège - ULg > Services généraux (Faculté de médecine) > Service administratif de la Faculté (Médecine) >]
Surlemont, C. [> > > >]
Vockel, A. [> > > >]
Harada, N. [> > > >]
Journal of Comparative Neurology
Yes (verified by ORBi)
[en] An immunocytochemical peroxidase-antiperoxidase procedure using a purified polyclonal antibody raised against human placental aromatase was used to localize aromatase-containing cells in the brain of three avian species: the Japanese quail, the ring dove, and the zebra finch. In quail and dove, immunoreactive cells were found only in the preoptic area and hypothalamus, with a high density of positive cells being present in the medial preoptic area, in the septal area above the anterior commissure, in the ventromedial nucleus of the hypothalamus, and in rostral part of the infundibulum. Immunoreactivity was weaker in zebra finches, and no signal could therefore be detected in the ventromedial and tuberal hypothalamus. The positive material was localized in the perikarya and in adjacent cytoplasmic processes, including the full length of axons always leaving a clear unstained cell nucleus. These features could be observed in more detail on sections cut from perfused brains and stained with an alkaline phosphatase procedure. The distribution of aromatase immunoreactivity was similar in the three species although minor differences were observed in the preoptic area. The localization of labelled neurons coincided with the distribution of aromatase activity as studied by in vitro radioenzyme assays on brain nuclei dissected by the Palkovits punch method. There was one striking exception to this rule: no immunoreactivity was detected in the zebra finch telencephalon, while assays had shown the presence of an active enzyme in several nuclei such as the robustus archistriatalis, the hyperstriatum ventrale pars caudale, and the hippocampus and area parahippocampalis. The origins of this discrepancy and the functional role of the aromatase observed in the axons are discussed.

File(s) associated to this reference

Fulltext file(s):

Restricted access
164_1990 BalthazartJCN.pdfPublisher postprint2.45 MBRequest copy

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.