Article (Scientific journals)
Isolation of Bovine Follicular Dendritic Cells Allows the Demonstration of a Particular Cellular Prion Protein
Thielen, Caroline; Mélot, France; Jolois, Olivier et al.
2001In Cell and Tissue Research, 306 (1), p. 49-55
Peer Reviewed verified by ORBi
 

Files


Full Text
Thielen et al 2001.pdf
Publisher postprint (481.44 kB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
follicular dendritic cells; lymphoid organs; proteolytic cleavage; prion protein; immunogold labelling; bovine
Abstract :
[en] As interaction of cellular prion protein (PrPc) and the infectious agent (PrPres) appears to be a crucial pathogenic step promoted by homology, variation in PrPc isoforms on bovine immune cells may explain the absence of infectivity in most bovine lymph organs. In this study, we examined PrPc expression in bovine lymph organs (tonsils and lymph nodes) and on isolated follicular dendritic cells (FDCs). We used a panel of different monoclonal antibodies (MoAbs) raised against different epitopes of prion protein. Two MoAbs recognise amino acids 79-92 (SAF 34 and SAF 32 Mo-Abs); the 6H4 antibody reacts with a specific peptide comprising the 144-152 amino acids, and the 12F10 MoAb recognises the sequence 142-160. After immunolabelling of frozen sections of lymph organs with 6H4 or 12F10 MoAbs, we detected cellular prion protein in germinal centres. However, using the SAF 34 or SAF 32 antibodies, PrPc was revealed outside the lymphoid tissues. No PrPc was observed in the germinal centres. Therefore, we adapted the method of FDC isolation, making it suitable for the study of PrPc expression on their surface. Using electron microscopy, the presence of PrPc on the surface of FDCs was demonstrated only with 6H4 MoAb. These results suggest that bovine follicular dendritic cells express a particular form of prion protein. Either the N-terminal part of PrPc is cleaved or the accessibility of the specific epitope (79-92) of SAF 34 MoAb is abolished by interaction with other molecules. This particular isoform of PrPc on bovine FDCs might be related to the apparent absence of infectivity in lymph organs in cattle affected by bovine spongiform encephalopathy.
Disciplines :
Immunology & infectious disease
Anatomy (cytology, histology, embryology...) & physiology
Author, co-author :
Thielen, Caroline ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie Humaine
Mélot, France ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie Humaine
Jolois, Olivier ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine
Leclercq, Frederic
Tsunoda, Rikiya
Frobert, Yveline
Heinen, Ernst ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine
Antoine, Nadine ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie Humaine
Language :
English
Title :
Isolation of Bovine Follicular Dendritic Cells Allows the Demonstration of a Particular Cellular Prion Protein
Publication date :
October 2001
Journal title :
Cell and Tissue Research
ISSN :
0302-766X
eISSN :
1432-0878
Publisher :
Springer, Germany
Volume :
306
Issue :
1
Pages :
49-55
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
FRIA - Fonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture [BE]
Available on ORBi :
since 18 December 2008

Statistics


Number of views
100 (12 by ULiège)
Number of downloads
1 (1 by ULiège)

Scopus citations®
 
16
Scopus citations®
without self-citations
11
OpenCitations
 
13

Bibliography


Similar publications



Contact ORBi