Reference : Isolation of Bovine Follicular Dendritic Cells Allows the Demonstration of a Particul...
Scientific journals : Article
Human health sciences : Immunology & infectious disease
Life sciences : Anatomy (cytology, histology, embryology...) & physiology
http://hdl.handle.net/2268/2836
Isolation of Bovine Follicular Dendritic Cells Allows the Demonstration of a Particular Cellular Prion Protein
English
Thielen, Caroline mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie Humaine > >]
Mélot, France mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie Humaine > >]
Jolois, Olivier mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie humaine >]
Leclercq, Frederic [ > > ]
Tsunoda, Rikiya [ > > ]
Frobert, Yveline [ > > ]
Heinen, Ernst mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie humaine >]
Antoine, Nadine mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie Humaine > >]
Oct-2001
Cell & Tissue Research
306
1
49-55
Yes (verified by ORBi)
International
0302-766X
[en] follicular dendritic cells ; lymphoid organs ; proteolytic cleavage ; prion protein ; immunogold labelling ; bovine
[en] As interaction of cellular prion protein (PrPc) and the infectious agent (PrPres) appears to be a crucial pathogenic step promoted by homology, variation in PrPc isoforms on bovine immune cells may explain the absence of infectivity in most bovine lymph organs. In this study, we examined PrPc expression in bovine lymph organs (tonsils and lymph nodes) and on isolated follicular dendritic cells (FDCs). We used a panel of different monoclonal antibodies (MoAbs) raised against different epitopes of prion protein. Two MoAbs recognise amino acids 79-92 (SAF 34 and SAF 32 Mo-Abs); the 6H4 antibody reacts with a specific peptide comprising the 144-152 amino acids, and the 12F10 MoAb recognises the sequence 142-160. After immunolabelling of frozen sections of lymph organs with 6H4 or 12F10 MoAbs, we detected cellular prion protein in germinal centres. However, using the SAF 34 or SAF 32 antibodies, PrPc was revealed outside the lymphoid tissues. No PrPc was observed in the germinal centres. Therefore, we adapted the method of FDC isolation, making it suitable for the study of PrPc expression on their surface. Using electron microscopy, the presence of PrPc on the surface of FDCs was demonstrated only with 6H4 MoAb. These results suggest that bovine follicular dendritic cells express a particular form of prion protein. Either the N-terminal part of PrPc is cleaved or the accessibility of the specific epitope (79-92) of SAF 34 MoAb is abolished by interaction with other molecules. This particular isoform of PrPc on bovine FDCs might be related to the apparent absence of infectivity in lymph organs in cattle affected by bovine spongiform encephalopathy.
Fonds pour la formation à la Recherche dans l'Industrie et dans l'Agriculture (Communauté française de Belgique) - FRIA
http://hdl.handle.net/2268/2836

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