[en] Attaching and effacing Escherichia coli are involved in diarrhea in 2 to 8-week old calves. The virulence factors of these bacteria include: (i) the secretion of proteins (i.e. EspB) involved in microvilli effacement, (ii) the production of the intimin, a 94 kDa outer membrane protein encoded by the eaeA gene and involved in the intimate attachment of bacteria to epithelial cell and (iii) the production of verotoxins: VT1 and/or VT2. We investigated the presence and the pathotype of these strains in several calf populations by colony hybridization or by genetic amplification. Using the colony hybridization method we showed first that only 5% of calves who died from diarrhea presented EaeA+ E. coli strains and secondly that 19% of healthy calves showed an asymptomatic carriage. However, using colony hybridization and genetic amplification, we identified EaeA+ strains in 91% of calves living in farms with recurrent diarrhea problems. In 66% of the calves, there was a correlation between the presence of AEEC and diarrhea. At the pathotype level, most of the EaeA+ isolates were negative for VT probes. In VT+ bacteria, the majority were VT1+. The number of VT positive bacteria was significantly higher in calves who died from diarrhea than in healthy or sick calves. This underlined the aggravating role of verotoxins in the disease. Moreover, only 25% of the bovine AEEC were positive with the EaeB probe. Surprisingly, the proportion of EaeB+ strains was significantly higher in healthy calves than in other populations.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
China, B.
Pirson, V.
Mainil, Jacques ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Bactériologie et pathologie des maladies bactériennes
Language :
English
Title :
Prevalence and Molecular Typing of Attaching and Effacing Escherichia Coli among Calf Populations in Belgium
Acres S.D. Enterotoxigenic E. coli infections in newborn calves: a review. J. Dairy Sci. 68:1985;229-256.
Atlas, R.M., 1993. In: Parks, L.C. (Ed.), Handbook of Microbiological Media. CRC Press, Boca Raton, pp. 523.
Butler, D.G., Clarke, R.C., 1994. Diarrhea and dysentery in calves. In: Gyles, C.L. (Ed.), Escherichia coli in domestic animals and humans. CAB international, Oxon, pp. 91-116.
Chanter N., Morgan J.H., Bridger J.C., Hall G.A., Reynolds D.R. Dysentery in gnotobiotic calves caused by atypical Escherichia coli. Vet. Rec. 114:1984;71.
China, B., Pirson, V., Jacquemin, E., Pohl, P., Mainil, J.G., 1997. Pathotypes of bovine verotoxygenic Escherichia coli isolates producing attaching and effacing (AE) lesion in ligated intestinal loop assay in rabbits. In: Francis, D., Benfield, D. (Eds.), Mechanisms in the Pathogenesis of Enteric Diseases. Plenum Press, New York, NY, pp. 311-316.
China B., Pirson V., Mainil J. Typing of bovine attaching and effacing Escherichia coli by multiplex in vitro amplification of virulence-associated genes. Appl. Environ. Microbiol. 62:1996;3462-3465.
Donnenberg, M.S., Tzipori, S., McKee, M.L., O'Brien, A.D., Alroy, J., Kaper, J.B., 1993a. The role of the eae gene of enterohemorrhagic Escherichia coli in intimate attachment in vitro and in porcine model. J. Clin. Invest. 92, 1418-1424.
Donnenberg, M.S., Yu, J., Kaper, J.B., 1993b. A second chromosomal gene necessary for intimate attachment of enteropathogenic Escherichia coli to epithelial cells. J. Bacteriol. 175, 4670-4680.
Goffaux F., Mainil J., Pirson V., Charlier G., Pohl P., Jacquemin E., China B. Bovine attaching and effacing Escherichia coli possess a pathogenesis island related to the LEE of the human enteropathogenic Escherichia coli strain E2348/69. FEMS Microbil. Let. 154:1997;415-421.
Jackson, M.P, Newland, J.W., Holmes, R.K., O'Brien, A.D. 1987a. Nucleotide sequence analysis of the structural genes for shiga-like toxin I encoded by bacteriophage 933J from Escherichia coli. Microb. Pathog. 2, 147-153.
Jackson, M.P., Neill, R.J., O'Brien, A.D., Holmes, R.K., Newland, J.W., 1987b. Nucleotide sequence analysis and comparison of the structural gene for Shiga-like toxin I and Shiga-like toxin II encoded by bacteriophages from Escherichia coli 933. FEMS Microbiol. Lett. 44, 109-114.
Jerse A.E., Yu J., Tall B.D., Kaper J.B. A genetic locus of enteropathogenic Escherichia coli necessary for the production of ataching and effacing lesions on tissue culture cells. Proc. Natl. Acad. Sci. USA. 87:1990;7839-7843.
Kenny B., Finlay B.B. Protein secretion by enteropathogenic Escherichia coli is essential for transducing signals to epithelial cells. Proc. Natl. Acad. Sci. USA. 92:1995;7991-7995.
Knutton, S., 1994. Attaching and effacing Escherichia coli. In: Gyles, C.L. (Ed.), Escherichia coli in Domestic Animals and Humans. CAB International, Oxon, pp. 567-91.
Konowalchuck J., Speirs J.L., Stavric S. Vero response to a cytotoxin of Escherichia coli. Infect. Immun. 18:1977;775-779.
Levine M.M. Escherichia coli that cause diarrhea: enterotoxigenic, enteropathogenic, enteroinvasive, enterohemorrhagic and enteroadherent. J. Infect. Dis. 155:1987;377-389.
Mainil J.G., Jacquemin E., Kaeckenbeeck A., Pohl P. Association between the effacing gene (eae) and the Shiga-like toxin-encoding genes in Escherichia coli isolates from cattle. Am. J. Vet. Res. 54:1993;1064-1068.
Moon H.W., Whipp S.C., Argenzio R.A., Levine M.M., Gianella R.A. Attaching and effacing activities of rabbit and human enteropathogenic Escherichia coli in pig and rabbit intestines. Infect. Immun. 53:1983;1340-1351.
Moseley S.L., Hardy J.M., Huq M.I., Echevarria P., Falkow S. Isolation and Nucleotide seuqence determiantion of a gene encoding a heat stable enterotoxin of Escherichia coli. Infect. Immun. 39:1983;1167-1174.
Newland J.W., Neill R.G. DNA probe for shiga-like toxins I and II and for toxin-converting bacteriophages. J. Clin. Microbiol. 26:1988;1292-1297.
O'Brien A.D., La Veck G.D. Purification and characterization of a Shigella dysenteriae 1-like toxin produced by Escherichia coli. Infect. Immun. 40:1983;675-683.
Pohjola, S., Jokipii, L., Jokipii, A. M.M., 1984. Dimethylsulphoxide-Ziehl-Neelsen staining technique for detection of cryptosporidial oocysts. The Veterinary Record, 115, 442-443.
So M., McCarthy B.J. Nucleotide sequence of transposon Tn1681 encoded a heat-stable toxin (ST) and its identification in enteroenterotoxigenic Escherichia coli strains. Proc. Nat. Acad. Sci. USA. 77:1980;4011-4015.
Tesch V.L., O'Brien A.D. The pathogenic mechanisms of shiga and Shiga-like toxins. Mol. Microbiol. 5:1991;1817-1822.
Tzipori, S., 1985. The relative importance of enteric pathogens affecting neonates of domestic animals. In: Cornelius, C.E., Simpson, C.F. (Eds.), Advances in Veterinary Science and Comparative Medicine, vol. 29. Academic Press, Toronto, pp. 103-206.