|Reference : Comparison of Eae, Tir, Espa and Espb Genes of Bovine and Human Attaching and Effacing E...|
|Scientific journals : Article|
|Life sciences : Veterinary medicine & animal health|
|Comparison of Eae, Tir, Espa and Espb Genes of Bovine and Human Attaching and Effacing Escherichia Coli by Multiplex Polymerase Chain Reaction|
|China, B. [> > > >]|
|Goffaux, F. [> > > >]|
|Pirson, V. [> > > >]|
|Mainil, Jacques [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Bactériologie et pathologie des maladies bactériennes >]|
|FEMS Microbiology Letters|
|Yes (verified by ORBi)|
|[en] Attaching and effacing Escherichia coli (AEEC) virulence genes include the eae, the tir, the espA and the espB genes. These genes have been sequenced from several AEEC strains. The sequences alignments revealed the presence of constant and variable regions. Multiplex polymerase chain reactions were developed, in order to determine the subtype of each gene present in a particular isolate. AEEC strains isolated from calves dead of diarrhea, from healthy calves and from infected humans were compared. The same pathotypes were found in sick and healthy calves but in inverted proportion. These pathotypes were also found in human AEEC. Although, the human EHEC strains from serotype O157 possessed their own pathotype.|
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