ORBi: Mignon Bernard - The in vitro and in vivo production of a 31.5-kD keratinolytic subtilase from Microsporum canis and the clinical status in naturally infected cats.

Reference : The in vitro and in vivo production of a 31.5-kD keratinolytic subtilase from Microsporu...


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Veterinary medicine & animal health
	To cite this reference:	http://hdl.handle.net/2268/28087

Title :	The in vitro and in vivo production of a 31.5-kD keratinolytic subtilase from Microsporum canis and the clinical status in naturally infected cats.
Language :	English
Author, co-author :	Mignon, Bernard [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Parasitologie et pathologie des maladies parasitaires >]
	Nikkels, Arjen [Centre Hospitalier Universitaire de Liège - CHU > > Dermatologie >]
	Pierard, Gérald [Centre Hospitalier Universitaire de Liège - CHU > > Dermatopathologie >]
	Losson, Bertrand [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Parasitologie et pathologie des maladies parasitaires >]
Publication date :	1998
Journal title :	Dermatology : International Journal for Clinical & Investigative Dermatology
Publisher :	S. Karger
Volume :	196
Issue/season :	4
Pages :	438-441
Audience :	International
ISSN :	1018-8665
e-ISSN :	1421-9832
City :	Basel
Country :	Switzerland
Keywords :	[en] Animals ; Cats ; Dermatomycoses/enzymology/microbiology/pathology ; Female ; Hair Follicle/drug effects/enzymology/microbiology ; Immunohistochemistry ; Keratins/metabolism ; Male ; Microsporum/chemistry/enzymology/isolation & purification ; Oligopeptides/pharmacology ; Phenylmethylsulfonyl Fluoride/pharmacology ; Protease Inhibitors/pharmacology ; Subtilisins/metabolism
Abstract :	[en] BACKGROUND: Microsporum-canis-infected cats, especially the asymptomatic infected ones, are mainly responsible for the zoonotic disease. The important variability of the clinical signs in cats is poorly understood. Recently, a 31.5-kD keratinolytic subtilase was found to be a putative virulence factor. OBJECTIVE: To investigate the possible relationship between the clinical status of dermatophytic cats and the production of the keratinase. METHODS: Seven M. canis strains isolated either from clinically affected, asymptomatic infected or mechanical carrier cats were tested for the in vitro production of the enzyme. The immunohistochemical detection of the enzyme was also assessed in skin biopsies of 4 symptomatic and 7 asymptomatic naturally infected cats. RESULTS: All the strains produced in vitro a 31.5-kD keratinolytic subtilase. The enzyme was present in all but 1 of the infected cats. CONCLUSION: The production of the keratinase is not a factor directly responsible for the clinical picture seen in M.-canis-infected cats.
Permalink :	http://hdl.handle.net/2268/28087

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