Histopathological pattern and humoral immune response to a crude exo-antigen and purified keratinase of Microsporum canis in symptomatic and asymptomatic infected cats.
[en] In order to understand better the mechanisms involved in the diverse clinical patterns in Microsporum canis-infected cats, the histopathological features were compared in symptomatic and asymptomatic infected cats. Additionally, the IgG immune response to a crude exo-antigen and purified keratinase of M. canis was studied by ELISA in cats of various clinical and mycological status. Acute and subacute perifolliculitis and folliculitis occurred more frequently in symptomatic than asymptomatic cats. The latter usually displayed signs of chronic inflammation and a marked infiltration of superficial dermis by mast cells, which would suggest that these animals present similarities to chronically dermatophytic humans or animals. When using a crude M. canis antigen, all infected cats were shown to have significantly higher levels of specific IgG when compared to culture negative and mechanical carrier-cats. In these non-infected animals, specific IgG was more frequently detected in adults than in young animals. No difference in anti-crude antigen specific IgG was observed between symptomatic and asymptomatic infected cats, indicating that the presence of IgG is probably unrelated to the clinical status of cats. Anti-keratinase specific IgG was only detected in one of the infected cats.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Mignon, Bernard ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Parasitologie et pathologie des maladies parasitaires
Coignoul, Freddy ; Université de Liège - ULiège > Département de morphologie et pathologie > Pathologie générale et autopsies
Leclipteux, T.
Focant, Charles ; Université de Liège - ULiège > Département des sciences de la vie > Biologie et génétique moléculaire
Losson, Bertrand ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Parasitologie et pathologie des maladies parasitaires
Language :
English
Title :
Histopathological pattern and humoral immune response to a crude exo-antigen and purified keratinase of Microsporum canis in symptomatic and asymptomatic infected cats.
Publication date :
1999
Journal title :
Medical Mycology
ISSN :
1369-3786
eISSN :
1460-2709
Publisher :
BIOS Scientific Publishers, Oxford, United Kingdom
Lunder M, Lunder M. Is Microsporum canis infection about to become a serious dermatological problem? Dermatology 1992; 184: 87-89.
Mignon BR, Losson BJ. Prevalence and characterization of Microsporum canis carriage in cats. J Med Vet Mycol 1997; 35: 249-256.
Gross TL, Ihrke PJ, Walder EJ. Feline dermatophytosis. In: Reinhardt RW, ed. Veterinary Dermatopathology. A macroscopic and microscopic evaluation of canine and feline skin disease, 1st edn., Mosby Year Book, Saint Louis, 1992; 241-243.
Mignon B, Swinnen M, Bouchara JP, Hofinger M, Nikkels A. Pierard Gerday Ch, Losson B. Purification and characterization of a 31.5 kDa keratinolytic subtilisin-like serine protease from Microsporum canis and evidence of its secretion in naturally infected cats. Med Mycol 1998; 36: 395-404.
Mignon BR, Nikkels AF, Piérard GE. Losson BJ. The in vitro and in vivo production of a 31.5 kDa keratinolytic subtilase from Microsporum canis and the clinical status in naturally infected cats. Dermatology 1998; 196: 438-441.
Grappel SF, Blank F. Role of keratinases in dermatophytosis. I. Immune responses of guinea pigs infected with Trichophyton mentagrophytes and guinea pigs immunized with keratinases. Dermatologica 1972; 45: 245-255.
Collins JP, Grappel SF, Blank F. Role of keratinases in dermatophytosis. II. Fluorescent antibody studies with keratinasa II of Trichophyton mentagrophytes. Dermatologica 1973; 146: 95-100.
Apodaca G, McKerrow JH. Purification and charaterization of a 27,000-Mr extracellular proteinase from Trichophyton rubrum. Infect Immun 1989; 57: 3072-3080.
Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976; 72: 248-254.
Scopes RK. Measurement of protein by spectrophotometry at 205 nm. Anal Biochem 1974; 59: 277-282.
Cassone A. Cell-mediated immunity mechanisms in fungal infections. In: Jacobs PH, Nall L, eds. Fungal Disease. Biology. Immunology and Diagnosis, 1st edn., Marcel Dekker, New York, 1997; 113-135.
Calderon RA, Hay RJ. Fungicidal activity of human neutrophils and monocytes in dermatophyte fungi Trichophyton quinckeanum and Trichophyton rubrum. Immunology 1987; 61: 289-295.
Hay RJ, Calderon RA, Mackenzie CD. Experimental dermatophytosis in mice: correlation between light and electron microscopic changes in primary, secondary, and chronic infection. Br J Exp Pathol 1988; 69: 703-716.
Wagner DK, Sonhle PG. Cutaneous defense mechanisms against fungi. In: Jacobs PH, Nall L, eds. Fungal Disease. Biology, Immunology and Diagnosis, 1st edn., Marcel Dekker, New York, 1997; 161-189.
Hay RJ, Calderon RA, Collins MJ. Experimental dermatophytosis: the clinical and histopathological features of a mouse model using Trichophyton quinckeanum (mouse favus). J Invest Dermatol 1983; 81: 270-274.
Kaaman T. Von Stedingk LV, Von Stedingk M, Wasserman J. ELISA-determined serological reactivity against purified trichophytin in dermatophytosis. Acta Derm Venereol 1981; 61: 313-317.
Sparkes AH, Stokes CR, Gruffydd-Jones TJ. Humoral immune response in cats with dermatophytosis. Am J Vet Res 1993; 54: 1869-1873.
De Boer DJ, Moriello KA. Humoral and cellular immune response to Microsporum canis in naturally occurring feline dermatophytosis. J Med Vet Mycol 1993; 31: 121-132.
Sparkes AH, Stokes CR, Gruffydd-Jones TJ. Experimental Microsporum canis infection in cats: correlation between immunological and clinical observations. J Med Vet Mycol 1995; 33: 177-184.
Sparkes AH, Gruffydd-Jones TJ, Stokes CR. Acquired immunity in experimental feline Microsporum canis infection. Res Vet Sci 1996; 61: 165-168.
Svejgaard E. Immunologic investigations of dermatophytes and dermatophytosis. Semin Dermatol 1985; 4: 201-221.
Honbo S, Jones HE, Artis WM. Chronic dermatophyte infection: evaluation of the Ig class-specific antibody response reactive with polysaccharide and peptide antigens derived from Trichophyton mentagrophytes. J Invest Dermatol 1984; 82: 287-290.
Lee KH, Kim YA, Lee MG, Lee JB. Detection of IgG and IgM antibodies to purified keratinolytic proteinase in sera from patients with dermatophytosis by enzyme-linked immunosorbent assay. Annal Dermatol 1989; 1: 1-6.
Lee KH, Lee JB, Song DH. Detection of circulating antibodies to purified keratinolytic proteinase in sera from guinea pigs infected with Microsporum canis by enzyme-linked immunosorbent assay. Arch Dermatol Res 1988; 280: 45-49.
DeBoer DJ, Moriello KA. The immune response to Microsporum canis induced by a fungal cell wall vaccine. Vet Dermatol 1994; 5: 47-56.
De Boer DJ, Moriello KA. Investigations of a killed dermatophyte cell-wall vaccine against infection with Microsporum canis in cats. Res Vet Sci 1995; 59: 110-113.
Slunt JB, Taketomi EA, Woodfolk JA, Hayden ML, Platts-Mills TAE. The immune response to Trichophyton tonsurans. Distinct T cell cytokine profiles to a single protein among subjects with immediate and delayed hypersensitivity. J Immunol 1996; 157: 5192-5197.
Hay RJ. Immunomodulation in superficial infections. In: Jacobs PH, Nall L, eds. Fungal Disease. Biology. Immunology and Diagnosis, 1st edn., Marcel Dekker, New York, 1997; 209-218.