fitness; Genetic diversity; natural and anthropogenic population
Abstract :
[en] We investigated variations in genetic diversity and plant fitness in a rare endemic metallophyte of calamine soils, Viola calaminaria, in relation to population size, population connectivity and population history in order to evaluate and discuss potential conservation strategies for the species. Mean population genetic diversity (Hs = 0.25) of V. calaminaria was similar to endemic non-metallophyte taxa. Twenty-one per cent of the genetic variation was partitioned among populations and a low (9%) but significant differentiation was found among geographical regions. Our results did not support the hypothesis that the acquisition of metal tolerance may result in reduced genetic diversity, and suggested that strict metallophytes do not exhibit higher inter-population differentiation resulting from scattered habitats. There were no relationships between population genetic diversity and population size. Significant correlations were found between plant fitness and (i) population size and (ii) connectivity index. Recently-founded populations exhibited the same level of genetic diversity as ancient populations and also possessed higher plant fitness. There was no indication of strong founder effects in recently-established populations. The results suggest that the creation of habitats through human activities could provide new opportunities for conservation of this species.
Antonovics J., Bradshaw A.D., Turner R.G. (1971) Heavy metal tolerance in plants. Advances in Ecological Research, 7, 1 85.
Baumbach H., Hellwig F.H. (2003) Genetic variation within and among metal-tolerant and non-tolerant populations of Armeria maritima (Mill.) Willd. s.l. (Plumbaginaceae) in Central and Northeast Germany. Plant Biology, 52, 186 193.
Bizoux J.-P. (2006) Biologie de la conservation d'une métallophyte endémique: Viola calaminaria. Ph.D. thesis, Faculté Universitaire des Sciences Agronomiques de Gembloux, Belgium.
Bizoux J.-P., Mahy G. (2007) Within-population genetic structure and clonal diversity of a threatened endemic metallophyte, Viola calaminaria (Violaceae). American Journal of Botany, 94, 887 895.
Bizoux J.-P., Brevers F., Meerts P., Graitson E., Mahy G. (2004) Ecology and conservation of Belgian populations of Viola calaminaria, a metallophyte with a restricted geographic distribution. Belgian Journal of Botany, 1371, 91 104.
Brock J., Aboling S., Stelzer R., Esch E., Papenbrock J. (2007) Genetic variation among different populations of Aster tripolium grown on naturally and anthropogenic salt-contaminated habitats: implications for conservation strategies. Journal of Plant Research, 120, 99 112.
Brown G. (2001) The heavy metal vegetation of north-western mainland Europe. Botanische Jahrbücher für Systematik, 1231, 63 110.
Broyles S.B., Schnabel A., Wyatt R. (1994) Evidence for long distance pollen dispersal in milkweeds (Asclepias exeltata). Evolution, 48, 1032 1040.
Charlesworth D., Charlesworth B. (1987) Inbreeding and its evolution consequences. Annual Review of Ecology and Systematics, 18, 237 268.
Dubois P.O., Cheptou C., Petit P., Meerts M., Poncelet X., Vekemans C., Lefèbvre C., Escarré J. (2003) Genetic structure and mating systems of metallicolous and nonmetallicolous populations of Thlaspi caerulescens. New Phytologist, 157, 633 641.
Ducousso A., Petit D., Valero M., Vernet P. (1990) Genetic variation between and within populations of a perennial grass: Arrenatherum elatius. Heredity, 65, 179 188.
Duvigneaud J. (1982) La halde calaminaire du Rocheux à Theux. Une nouvelle réserve naturelle d'Ardenne et Gaume. Parc nationaux, 37, 117 138.
Duvigneaud J., Saintenoy-Simon J. (1996) Le site calaminaire de Plombières. Ardenne et Gaume, 5101, 5 9.
Ellstrand N.C., Elam D.R. (1993) Population genetic consequences of small population size - Implications for plant conservation. Annual Review of Ecology and Systematics, 24, 217 242.
Ernst W.H.O. (1990) Mine vegetation in Europe. In: Shaw A.J. (Ed. Heavy Metal Tolerance in Plants: Evolutionary Aspects. CRC Press, Boca Raton, FL: 285 300.
Excoffier L., Smouse P.E., Quattro M. (1992) Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics, 131, 479 491.
Fischer M., Matthies D. (1998) RAPD variation in relation to population size and plant fitness in the rare Gentianella germanica (Gentianaceae). American Journal of Botany, 856, 811 819.
Hadrys H., Blick M., Schierwater B. (1992) Applications of random amplified polymorphic DNA (RAPD) in molecular ecology. Molecular Ecology, 1, 55 63.
Hamrick J.L., Godt M.J.W. (1996) Effects of life history traits on genetic diversity in plant species. Philosophical Transactions of the Royal Society of London Series B-Biological Sciences, 351, 1291 1298.
Hanski I. (1994) A practical model of metapopulation dynamics. Journal of Animal Ecology, 63, 151 162.
Heimans J. (1961) Taxonomical, phytogeographical and ecological problems round Viola calaminaria, the zinc violet. Publicaties Natuurhist Genootschap Limburg, 12, 55 71.
Hensen I., Oberprieler C. (2005) Effects of population size on genetic diversity and seed production in the rare Dictamnus albus (Rutaceae) in central Germany. Conservation Genetics, 61, 63 73.
Hensen I., Oberprieler C., Wesche K. (2005) Genetic structure, population size, and seed production of Pulsatilla vulgaris Mill. (Ranunculaceae) in Central Germany. Flora, 200, 3 14.
Hildebrandt U., Hoef-Emden K., Backhausen S., Bothe H., Bozek M., Siuta A., Kuta E. (2006) The rare, endemic zinc violets of Central Europe originate from Viola lutea Huds. Plant Systematics and Evolution, 257, 205 222.
Holsinger K.E., Wallace L.E. (2004) Bayesian approaches for the analysis of population genetic structure: an example from Platanthera leucophaea (Orchidaceae). Molecular Ecology, 134, 887 894.
Holsinger K.E., Lewis P.O., Dey D.K. (2002) A Bayesian approach to inferring population structure from dominant markers. Molecular Ecology, 11, 1157 1164.
Kakes P. (1977) Genecological investigations on zinc plants II. Introgression in a small population of the zinc violet Viola calaminaria subsp. westfalica Lej. Ernst. Acta Botanica Neerlandica, 26, 385 400.
Kakes P. (1979) Genecological investigations on zinc plants III. Cytology of hybrids between Viola calaminaria (Lej.) Ernst and Viola arvensis Murr. Genetica, 512, 135 142.
Krahulcova A., Krahulec F., Kirschner J. (1996) Introgressive hybridization between a native and an introduced species: Viola lutea subsp. sudetica versus V. tricolor. Folia Geobotanica et Phytotaxonomica, 312, 219 244.
Krüger A.M., Hellwig F.H., Oberprieler C. (2002) Genetic diversity in natural and anthropogenic inland populations of salt-tolerant plants: random amplified polymorphic DNA analyses of Aster tripolium L. (Compositae) and Salicornia ramosissima Woods (Chenopodiaceae). Molecular Ecology, 119, 1647 1655.
Lambinon J., Auquier P. (1964) La flore et la végétation des terrains calaminaires de la Wallonie septentrionale et de la Rhénanie aixoise. Types chorologiques et groupes écologiques. Natura Mosana, 16, 113 130.
Lande R. (1988) Genetics and demography in biological conservation. Science, 241, 1455 1460.
Leimu R., Mutikainen P. (2005) Population history, mating system, and fitness variation in a perennial herb with a fragmented distribution. Conservation Biology, 192, 349 356.
Lynch M., Milligan B.G. (1994) Analysis of population genetic structure with RAPD markers. Molecular Ecology, 32, 91 99.
Mattner J., Zawko G., Rossetto M., Krauss S.L., Dixon K.W., Sivasithamparam K. (2002) Conservation genetics and implications for restoration of Hemigenia exilis (Lamiaceae), a serpentine endemic from Western Australia. Biological Conservation, 1071, 37 45.
McCauley D.E., Raveill J., Antonovics J. (1995) Local founding events as determinants of genetic structure in a plant metapopulation. Heredity, 75, 630 636.
Mengoni A., Gonnelli C., Galardi F., Gabbrielli R., Bazzicalupo M. (2000) Genetic diversity and heavy metal tolerance in populations of Silene paradoxa L. (Caryophyllaceae): a random amplified polymorphic DNA analysis. Molecular Ecology, 99, 1319 1324.
Nei M., Maruyama T., Chakraborty R. (1975) The bottleneck effect and genetic variability in populations. Evolution, 29, 1.
Neuffer B., Auge H., Mesch H., Amarell U., Brandl R. (1999) Spread of violets in polluted pine forests: morphological and molecular evidence for the ecological importance of interspecific hybridization. Molecular Ecology, 83, 365 377.
Nybom H., Bartish I. (2000) Effects of life history traits and sampling strategies on genetic diversity estimates obtained with RAPD markers in plants. Perspectives in Plant Ecology, Evolution and Systematics, 32, 93 114.
Oostermeijer J.G.B., Luijten S.H., den Nijs J.C.M. (2003) Integrating demographic and genetic approaches in plant conservation. Biological Conservation, 1133, 389 398.
Pluess A.R., Stöcklin J. (2004) Population genetic diversity of the clonal plant Geum reptans (Rosaceae) in the Swiss Alps. American Journal of Botany, 9112, 2013 2021.
Price M.V., Waser N.M. (1979) Pollen dispersal and optimal outcrossing in Delphinium nelsonii. Nature, 277, 294 296.
Quintana-Ascencio P.F., Weekley C.W., Menges E.S. (2007) Comparative demography of a rare species in Florida scrub and road habitats. Biological Conservation, 137, 263 270.
Rathcke B.J., Jules E.S. (1993) Habitat fragmentation and plant pollinator interactions. Current Science, 65, 273 277.
Reed D.H. (2005) Relationship between population size and fitness. Conservation Biology, 19, 563 568.
Reed D.H., Frankham R. (2003) Correlation between fitness and genetic diversity. Conservation Biology, 171, 230 237.
Schemske D.W., Lande R. (1985) The evolution of self-fertilization and inbreeding depression in plants. II. Empirical observations. Evolution, 39, 41 52.
Schneider S., Roessli D., Excoffier L. (2000) Arlequin ver 2.000: A Software for Population Genetics Data Analysis. Genetics and Biometry Laboratory, University of Geneva, Geneva.
Sih A., Baltus M.S. (1987) Patch size, pollinator behavior, and pollinator limitation in catnip. Ecology, 686, 1679 1690.
Slatkin M. (1977) Gene flow and genetic drift in a species subject to frequent extinctions. Theoretical Population Biology, 12, 253 262.
Vekemans X., Lefèbvre C. (1997) On the evolution of heavy-metal tolerant populations in Armeria maritima: Evidence from allozyme variation and reproductive barriers. Journal of Evolutionary Biology, 102, 175 191.
Wade M.J., McCauley D.E. (1988) Extinction and recolonization - their effects on the genetic differentiation of local populations. Evolution, 425, 995 1005.
Westerbergh A., Saura A. (1992) The effect of serpentine on the population structure of Silene dioica (Caryophyllaceae). Evolution, 46, 1537 1548.
Whiting S.N., Reeves R.D., Richards D., Johnson M.S., Cooke J.A., Malaisse F., Paton A., Smith J.A.C., Angle J.S., Chaney R.L., Ginocchio R., Jaffre T., Johns R., McIntyre T., Purvis O.W., Salt D.E., Schat H., Zhao F.J., Baker A.J.M. (2004) Research priorities for conservation of metallophyte biodiversity and their potential for restoration and site remediation. Restoration Ecology, 121, 106 116.
Wolf A. (2001) Conservation of endemic plants in serpentine landscapes. Biological Conservation, 1001, 35 44.
Wolf A.T., Harrison S.P. (2001) Effects of habitat size and patch isolation on reproductive success of serpentine morning glory. Conservation Biology, 15, 111 121.
Wolf A.T., Howe R.W., Hamrick J.L. (2000) Genetic diversity and population structure of the serpentine endemic Calystegia collina (Convolvulaceae) in northern California. American Journal of Botany, 87, 1138 1146.
