Reference : Effect of ppMCH derived peptides on PBMC proliferation and cytokine expression
Scientific journals : Article
Life sciences : Anatomy (cytology, histology, embryology...) & physiology
Human health sciences : Endocrinology, metabolism & nutrition
http://hdl.handle.net/2268/27599
Effect of ppMCH derived peptides on PBMC proliferation and cytokine expression
English
Coumans, Bernard mailto [Université de Liège - ULg > > CNCM/ Centre fac. de rech. en neurobiologie cell. et moléc. >]
Grisar, Thierry mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Biochimie et physiologie humaine et pathologique]
Nahon, J. L. [> > > >]
Lakaye, Bernard mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Biochimie et physiologie humaine et pathologique >]
4-Oct-2007
Regulatory Peptides
Elsevier Science Bv
143
1-3
104-108
Yes (verified by ORBi)
International
0167-0115
Amsterdam
[en] prepro-MCH (ppMCH) ; melanin concentrating hon-none (MCH) ; neuropeptide gluramic acid-isoleucine (NEI) ; neuropeptide glycine-glutamic acid (NGE) ; peptide MCH-NEl ; peripheral blood mononuclear cell (PBMC) ; cytokines
[en] The mRNA encoding prepro-Melanin concentrating hormone (ppMCH) is mainly expressed in the central nervous system but has also been detected at lower amount in many peripheral tissues including spleen and thymus. At the peptide level however, several forms of the precursor can be detected in these tissues and are sometimes expressed at similar levels compared to brain. In the present work, we have studied the in vitro action of a wide range of concentration (1 nM to 1 microM) of the different peptides encoded by ppMCH i.e. neuropeptide glycine-glutamic acid (NGE), neuropeptide glutamic acid-isoleucine (NEI), Melanin concentrating hormone (MCH) and the dipeptide NEI-MCH on peripheral blood mononuclear cells (PBMC) proliferation and cytokine production following anti-CD3 stimulation. Among them only MCH decreased PBMC proliferation with a maximal effect of 35% at 100 nM. Moreover as demonstrated by using ELISA, MCH significantly decreases IL-2 production by 25% but not IL-4, INF-gamma or TNF-alpha expression. Interestingly, exogenous IL-2 decreases significantly MCH-mediated inhibition, suggesting that it is an important downstream mediator of MCH action. Finally, we showed that after 7 to 9 days of incubation, MCH also inhibits proliferation of non-stimulated PBMC. Altogether, these data demonstrate that fully mature MCH modulates proliferation of anti-CD3 stimulated PBMC partially through regulation of IL-2 production.
http://hdl.handle.net/2268/27599
10.1016/j.regpep.2007.04.001

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