Reference : Adhesion and growth inhibitory effect of chicken egg yolk antibody (IgY) on Salmonell...
Scientific journals : Article
Life sciences : Veterinary medicine & animal health
Life sciences : Animal production & animal husbandry
Life sciences : Microbiology
http://hdl.handle.net/2268/27564
Adhesion and growth inhibitory effect of chicken egg yolk antibody (IgY) on Salmonella enterica serovars Enteritidis and Typhimurium in vitro.
English
Chalghoumi, Raja [> > > >]
Thewis, André mailto [Université de Liège - ULg > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech >]
Beckers, Yves mailto [Université de Liège - ULg > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech >]
Marcq, Christopher mailto [Université de Liège - ULg > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech >]
Portetelle, Daniel mailto [Université de Liège - ULg > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech >]
Schneider, Yves-Jacques [Université Catholique de Louvain - UCL > Institut des Sciences de la Vie > Biochimie cellulaire, nutritionnelle et toxicologique > >]
2009
Foodborne Pathogens and Disease
6
5
593-604
Yes (verified by ORBi)
International
1535-3141
1556-7125
[en] Immunoglobulin Y ; Salmonella spp. ; in vitro adhesion ; Caco-2 cells ; in vitro growth
[en] The protective effects of powder preparation of egg yolk immunoglobulin Y (IgY), specific to Salmonella Enteritidis and Salmonella Typhimurium outer membrane proteins (OMP), against these two Salmonella sp. serovars were investigated in vitro in two different assays: adhesion-prevention and growth-inhibition. The adhesion-prevention assay was conducted using polarized monolayers of the human intestinal epithelial Caco-2 cell line. First, the conditions of Salmonella adherence to Caco-2 cells were optimized, and interferences of bacteria with the transepithelial electrical resistance (TER) of fully differentiated Caco-2 cell monolayers and the lactate dehydrogenase release upon exposure of the cells to Salmonella were evaluated. Both Salmonella sp. serovars were able to adhere to Caco-2 cells and decreased TER. Results from the adhesion-prevention assay demonstrated that specific IgY reduced the decrease in TER of the infected Caco-2 cell monolayers and blocked the Salmonella sp. adhesion in a concentration-dependent manner (p < 0.05). Nonspecific IgY also exhibited an inhibitory effect on these two parameters, but to a lesser extent than that of the specific IgY (p < 0.05). The protective effect of nonspecific IgY could be attributed to the low-density lipoprotein component of the water-soluble fraction of egg yolks that may not have been eliminated during ultrafiltration. The growth-inhibition assay revealed that specific IgY had an inhibitory effect on the bacterial growth, markedly during the late exponential phase, whereas nonspecific IgY failed to do so. Taken together, these results suggest that the in vitro growth inhibitory effect of specific IgY on Salmonella spp. resulted from the specific binding activity of these IgY to Salmonella sp. OMP. Passive immunization with Salmonella sp. OMP-specific IgY could thus be useful to prevent Salmonella colonization in broiler chickens and the subsequent carcass contamination during processing.
Fonds pour la formation à la Recherche dans l'Industrie et dans l'Agriculture (Communauté française de Belgique) - FRIA ; Région wallonne : Direction générale de l'Agriculture - DGA
Développement et utilisation des anticorps du jaune d'oeuf en vue du contrôle des Salmonella spp. dans le tube digestif du poulet de chair
Researchers
http://hdl.handle.net/2268/27564
10.1089/fpd.2008.0258

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