Article (Scientific journals)
Effects of systemic versus local gentamicin on the inner ear in the Atlantic cod, Gadus morhua (L.), relevance for fish hearing investigations.
Faucher, Karine; Aas-Hansen, Øyvind; Damsgard, B. et al.
2008In Hearing Research, 240 (1-2), p. 12-21
Peer Reviewed verified by ORBi
 

Files


Full Text
Faucher et al 2008b.pdf
Publisher postprint (1.31 MB)
Request a copy
Full Text Parts
Corrected proofs.pdf
Author postprint (1.31 MB)
Download

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
Animals; Anti-Bacterial Agents/administration & dosage/toxicity; Dose-Response Relationship, Drug; Ear, Inner/drug effects/physiopathology/ultrastructure; Gadus morhua; Gentamicins/administration & dosage/toxicity; Hair Cells, Auditory/drug effects; Hearing/drug effects; Injections, Intravenous; Kidney/drug effects; Microscopy, Electron, Scanning; Models, Animal; Time Factors
Abstract :
[en] Fish models are increasingly being used for hearing research investigations. Aminoglycoside antibiotics that are used for damaging the inner ear hair cells can have systemic side effects leading to death of study animals. This study aimed to compare two methods: (i) systemic (intravenous) and (ii) local (intrasaccular) gentamicin administration for induction of inner ear hair cell damage in the Atlantic cod, Gadus morhua (L.). Hair cell damage was assessed using scanning electron microscopy; hair cell density, prevalence of immature hair cells and kinocilia length were measured. Gentamicin-treated fish were compared with control and sham fish. Intravenous gentamicin led to dose-dependent mortality caused by nephrotoxicity. The only visible effect after treatment was more immature hair cells and shorter kinocilia, the effect on hair cell density was equivocal. Following intrasaccular gentamicin treatment, fish mortality was negligible, and hair cells were damaged regardless of dose. Here, we observed decreased hair cell density, high prevalence of immature hair cells, and significantly shortened kinocilia. Conclusion: intrasaccular injection is preferable to intravenous injection of gentamicin for the study of ototoxicity in the Atlantic cod.
Disciplines :
Aquatic sciences & oceanology
Author, co-author :
Faucher, Karine ;  Université de Liège - ULiège > Département des sciences et gestion de l'environnement > Morphologie fonctionnelle et évolutive
Aas-Hansen, Øyvind
Damsgard, B.
Stenklev, N. C.
Language :
English
Title :
Effects of systemic versus local gentamicin on the inner ear in the Atlantic cod, Gadus morhua (L.), relevance for fish hearing investigations.
Publication date :
2008
Journal title :
Hearing Research
ISSN :
0378-5955
eISSN :
1878-5891
Publisher :
Elsevier Science, Amsterdam, Netherlands
Volume :
240
Issue :
1-2
Pages :
12-21
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 04 November 2009

Statistics


Number of views
103 (7 by ULiège)
Number of downloads
271 (3 by ULiège)

Scopus citations®
 
11
Scopus citations®
without self-citations
9
OpenCitations
 
11

Bibliography


Similar publications



Contact ORBi