Reference : The in vitro influences of neurotensin on the motility characteristics of human U373 gli...
Scientific journals : Article
Social & behavioral sciences, psychology : Neurosciences & behavior
Human health sciences : Neurology
http://hdl.handle.net/2268/27501
The in vitro influences of neurotensin on the motility characteristics of human U373 glioblastoma cells
English
Servotte, S. [Université de Liège - ULG > Département des Sciences Biomédicales et Précliniques > Laboratoire de Biologie des Tissus Conjonctifs > >]
Camby, I. [Université Libre de Bruxelles - ULB > Institut de Pharmacie > Laboratoire de Toxicologie > >]
Debeir, O. [Université Libre de Bruxelles - ULB > Department of Logical and Numerical Systems > Faculty of Applied Science > >]
Deroanne, Christophe mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Protéines et glycoprot. de matr.extracell. et membran.basal. >]
Lambert, C. mailto [Université de Liège - ULG > Département des Sciences biomédicales et précliniques > Laboratoire de Biologie des Tissus Conjonctifs > >]
Lapiere, C. M. [Université de Liège - ULG > > > > > >]
Kiss, R. [Université Libre de Bruxelles - ULB > Institut de Pharmacie > Laboratoire de Toxicologie > >]
Nusgens, Betty mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Protéines et glycoprot. de matr.extracell. et membran.basal. >]
Decaestecker, C. [Université Libre de Bruxelles - ULB > Institut de Pharmacie > Laboratoire de Toxicologie > >]
Dec-2006
Neuropathology & Applied Neurobiology
Blackwell Publishing
32
6
575-584
Yes (verified by ORBi)
International
0305-1846
Oxford
[en] cell migration ; glioblastoma ; neurotensin ; Rho GTPases ; scratch wound ; videomicroscopy
[en] Astrocytic tumours are associated with dismal prognoses due to their pronounced ability to diffusely invade the brain parenchyma. Various neuropeptides, including gastrin, are able to modulate tumour astrocyte migration. While neurotensin has been shown to influence the proliferation of glioma cells and the migratory ability of a large set of other cell types, its role in glioma cell migration has never been investigated. Neurotensin-induced modifications to the motility features of human U373 glioblastoma cells therefore constitute the topic of the present study. We evidenced that three subtypes of neurotensin receptors (NTR1, NTR2 and NTR3) are expressed in U373 glioblastoma cells, at least as far as their mRNAs are concerned. Treating U373 tumour cells with 10 nM neurotensin markedly modified the morphological patterns of these cells and also profoundly altered the organization of their actin cytoskeletons. Pull-down assays revealed that neurotensin induced the activation in U373 cells of both Rac1 and Cdc42 but not RhoA. Scratch wound assays evidenced that neurotensin (0.1 and 10 nM) very significantly inhibited wound colonization by U373 cells cultured in the absence of serum. In addition, quantitative phase-contrast videomicroscopy analyses showed that neurotensin decreases the motility levels of U373 glioblastoma cells when these cells are cultured on plastic. In sharp contrast, neurotensin stimulates the motility of U373 cells when they are cultured on laminin, which is a pro-adhesive extracellular matrix component ubiquitously secreted by glioma cells. Our data thus strongly suggest that, in addition to gastrin, neurotensin is a neuropeptide capable of modulating tumour astrocyte migration into the brain parenchyma.
Researchers
http://hdl.handle.net/2268/27501
The definitive version is available at www.blackwell-synergy.com

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