Reference : Airway Mast-Cell Activation in Asthmatics Is Associated with Selective Sputum Eosinophilia
Scientific journals : Article
Human health sciences : Cardiovascular & respiratory systems
http://hdl.handle.net/2268/27445
Airway Mast-Cell Activation in Asthmatics Is Associated with Selective Sputum Eosinophilia
English
Bettiol, Jane [> > > >]
Radermecker, Maurice [Université de Liège - ULg > > Pneumologie-Allergologie >]
Sele, Jocelyne mailto [Centre Hospitalier Universitaire de Liège - CHU > > Pneumologie-Allergologie >]
Henquet, Monique [> > > >]
Cataldo, Didier mailto [Université de Liège - ULg > Département des sciences cliniques > Labo de biologie des tumeurs et du développement - Département des sciences biomédicales et précliniques]
Louis, Renaud mailto [Université de Liège - ULg > Département des sciences cliniques > Pneumologie - Allergologie]
Nov-1999
Allergy
Blackwell Publishing
54
11
1188-93
Yes (verified by ORBi)
International
0105-4538
1398-9995
Oxford
United Kingdom
[en] asthma ; eosinophils ; neutrophils ; sputum ; sputum
[en] BACKGROUND: Tryptase is a serine endoprotease selectively released from mast cells. Although mast cells are known to be activated after experimental allergic provocation, their role in naturally occurring asthma is still debated. METHODS: We have investigated the levels of tryptase in the whole induced sputum collected from 51 asthmatics (31 atopic and 20 intrinsic) seen in our outpatient clinic and 22 normal nonatopic healthy volunteers. Tryptase was measured by a new immunoassay based on B12 monoclonal antibody recognition of total tryptase (UniCAP System, Pharmacia) with a sensitivity of 1 ng/ml. RESULTS: While being below the threshold of detection in all normal volunteers, tryptase was detectable in the sputum from 9/51 asthmatics (18%) including five atopic and four intrinsic asthma cases. In these patients, among whom three were asymptomatic asthmatics, the values ranged between 1 and 6.1 ng/ml. The asthmatics with detectable sputum tryptase had greater sputum eosinophil counts (P<0.05) but lower neutrophil counts (P<0.05) than those in whom tryptase was undetectable. When compared to control subjects, asthmatics without tryptase had still greater eosinophil counts (P<0.0001) but also raised neutrophil counts (P<0.05). No significant difference could be found between asthmatics with tryptase and those without tryptase with respect to the age, the baseline lung function, the methacholine bronchial responsiveness, and the frequency of treatment with inhaled steroids. CONCLUSIONS: With the UniCAP System, tryptase was detectable in the sputum from 18% of asthmatics irrespective of atopy and current symptoms. Asthmatics with tryptase appeared to have a selective increase in sputum eosinophil counts while those without tryptase displayed a mixed sputum granulocyte infiltration with raised eosinophil and neutrophil counts.
http://hdl.handle.net/2268/27445
10.1034/j.1398-9995.1999.00177.x
http://www3.interscience.wiley.com/journal/119069407/issue

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