Article (Scientific journals)
Separation and identification of endoxylanases from Bacillus subtilis and their actions on wheat bran insoluble dietary fibre
Xiaoping, Yuan; Jing, Wang; Huiyuan, Yao et al.
2005In Process Biochemistry, 40 (7), p. 2339-2343
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Keywords :
Bacillus subtilis; Electrophoresis; Endoxylanase; Purification; Wheat bran
Abstract :
[en] A novel and convenient method based on native polyacrylamide gel electrophoresis (PAGE) and homogenization extraction was used for the purification of xylanase from crude enzymes. Two xylanases were purified by this method from the crude enzyme preparation from the selected strain of Bacillus subtilis. Subsequent analysis with thin layer chromatography and high pressure liquid chromatography (HPLC) indicated that these two xylanases were endo-acting enzymes, designated xyl I and xyl II. Both enzymes showed their activities in the pH range from 5.0 to 9.0 at 50 °C and had similar optimum activities at pH 7.0 and at 50 °C. Mn2+ ions enhanced their xylanolytic activities to 2.7-fold whereas Fe3+ completely inhibited them. The action of endoxylanase xyl II on wheat bran insoluble dietary fibre was also studied. The hydrolysis products were shown to contain feruloyl oligosaccharides by paper chromatography
Disciplines :
Food science
Author, co-author :
Xiaoping, Yuan;  School of Food Science and Technology, Southern Yangtze University, 170 Hui He Road, Wuxi 214036, JiangSu Province, PR China > School of Food Science and Technology, Southern Yangtze University, 170 Hui He Road, Wuxi 214036, JiangSu Province, PR China
Jing, Wang;  School of Food Science and Technology, Southern Yangtze University, 170 Hui He Road, Wuxi 214036, JiangSu Province, PR China > School of Food Science and Technology, Southern Yangtze University, 170 Hui He Road, Wuxi 214036, JiangSu Province, PR China
Huiyuan, Yao;  School of Food Science and Technology, Southern Yangtze University, 170 Hui He Road, Wuxi 214036, JiangSu Province, PR China > School of Food Science and Technology
Nihorimbere, Venant ;  Université de Liège - ULiège > Doct. sc. agro. & ingé. biol.
Language :
English
Title :
Separation and identification of endoxylanases from Bacillus subtilis and their actions on wheat bran insoluble dietary fibre
Publication date :
2005
Journal title :
Process Biochemistry
ISSN :
1359-5113
Publisher :
Elsevier Science, Oxford, United Kingdom
Volume :
40
Issue :
7
Pages :
2339-2343
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 30 October 2009

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