Reference : Adherence of Mycoplasma bovis to bovine bronchial epithelial cells
Scientific journals : Article
Human health sciences : Immunology & infectious disease
Life sciences : Microbiology
http://hdl.handle.net/2268/26835
Adherence of Mycoplasma bovis to bovine bronchial epithelial cells
English
Thomas, Anne [> > > >]
Sachse, K. [> > > >]
Farnir, Frédéric mailto [Université de Liège - ULg > Département de productions animales > Biostatistique, économie, sélection animale >]
Dizier, Isabelle [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Bactériologie et pathologie des maladies bactériennes >]
Mainil, Jacques mailto [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Bactériologie et pathologie des maladies bactériennes >]
Linden, Annick mailto [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Santé et pathologies de la faune sauvage >]
Mar-2003
Microbial Pathogenesis
Academic Press Ltd Elsevier Science Ltd
34
3
141-148
Yes (verified by ORBi)
International
0882-4010
London
United Kingdom
[en] Mycoplasma bovis ; cytadherence ; primary culture ; bronchial cells ; variable surface lipoproteins
[en] Mycoplasma bovis is responsible for considerable economic losses in cattle due to pneumonia, arthritis and mastitis. As the agent was shown to be capable of adhering to neutrophils and embryonic bovine lung (EBL) cells and invading the respiratory epithelium it is highly desirable to improve our understanding of cytadherence processes. Although several surface proteins likely to be directly involved in this initial stage of interaction between pathogen and host cells have been identified, these findings mainly referred to type strain PG45 adhering to the continuous EBL cell line. The present study provides new and complementary data about cytadherence of M. bovis based on adherence of various radiolabeled strains to a primary culture of bovine bronchial epithelial (BBE) cells using a standardized adherence assay. M. bovis was shown to adhere specifically to the primary culture of BBE cells. Inhibition of adherence was observed upon addition of monoclonal antibodies (MAbs), trypsin treatment of mycoplasmas, and competition with non-radiolabeled mycoplasma cells. Interestingly, three MAbs against proteins involved in adherence to EBL cells failed to inhibit significantly the adherence to BBE cells. On the other hand, significant reduction of adherence rates by MAbs 2A8 and 9F1 directed against epitopes of variable surface lipoproteins VspC and VspF, respectively, demonstrated the involvement of these proteins in adherence of M. bovis to primary culture of BBE cells. (C) 2003 Elsevier Science Ltd. All rights reserved.
http://hdl.handle.net/2268/26835
also: http://hdl.handle.net/2268/71682

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