Article (Scientific journals)
Enteropathogenic and enterohaemorrhagic Escherichia coli deliver a novel effector called Cif, which blocks cell cycle G(2)/M transition
Marches, O.; Ledger, T. N.; Boury, M. et al.
2003In Molecular Microbiology, 50 (5), p. 1553-1567
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Abstract :
[en] Enteropathogenic Escherichia coli (EPEC) and enterohaemorrhagic E. coli (EHEC) are closely related pathogens. Both use a type III secretion system (TTSS) encoded by the 'locus of enterocyte effacement' (LEE) to subvert and attach to epithelial cells through the injection of a repertoire of effector molecules. Here, we report the identification of a new TTSS translocated effector molecule called Cif, which blocks cell cycle G(2)/M transition and induces the formation of stress fibres through the recruitment of focal adhesions. Cif is not encoded by the LEE but by a lambdoid prophage present in EPEC and EHEC. A cif mutant causes localized effacement of microvilli and intimately attaches to the host cell surface, but is defective in the ability to block mitosis. When expressed in TTSS competent LEE-positive pathogens, Cif is injected into the infected epithelial cells. These cells arrested at the G(2)/M phase displayed accumulation of inactive phosphorylated Cdk1. In conclusion, Cif is a new member of a growing family of bacterial cyclomodulins that subvert the host eukaryotic cell cycle.
Disciplines :
Microbiology
Biochemistry, biophysics & molecular biology
Author, co-author :
Marches, O.
Ledger, T. N.
Boury, M.
Ohara, M.
Tu, X. L.
Goffaux, F.
Mainil, Jacques ;  Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Bactériologie et pathologie des maladies bactériennes
Rosenshine, I.
Sugai, M.
De Rycke, J.
Oswald, E.
Language :
English
Title :
Enteropathogenic and enterohaemorrhagic Escherichia coli deliver a novel effector called Cif, which blocks cell cycle G(2)/M transition
Publication date :
December 2003
Journal title :
Molecular Microbiology
ISSN :
0950-382X
eISSN :
1365-2958
Publisher :
Blackwell Publishing Ltd, Oxford, United Kingdom
Volume :
50
Issue :
5
Pages :
1553-1567
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 27 October 2009

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