|Reference : Release from inflamed tissue of a substance with properties similar to corticotropin-rel...|
|Scientific journals : Article|
|Human health sciences : Anesthesia & intensive care|
|Release from inflamed tissue of a substance with properties similar to corticotropin-releasing factor.|
|Hargreaves, K. M. [> > > >]|
|Costello, A. H. [> > > >]|
|Joris, Jean [Centre Hospitalier Universitaire de Liège - CHU > > Anesthésie et réanimation >]|
|Yes (verified by ORBi)|
|[en] Animals ; Calcium/pharmacology ; Carrageenan ; Chromatography, High Pressure Liquid ; Corticotropin-Releasing Hormone/metabolism ; Denervation ; Hindlimb/innervation ; Inflammation/chemically induced/metabolism ; Male ; Radioimmunoassay ; Rats ; Rats, Inbred Strains ; beta-Endorphin/metabolism|
|[en] In response to stressors involving tissue injury, pituitary corticotroph secretion of immunoreactive beta-endorphin (iB-END) could be either due to release of hypothalamic factors such as corticotropin-releasing factor (CRF) or to release of a tissue factor from the periphery. In the present experiments, we investigated whether inflamed tissue releases a factor which evokes pituitary secretion of iB-END. In an initial experiment, rats with an inflamed hindpaw due to carrageenan injection had significantly greater levels of circulating iB-END as compared to rats with saline-injected paws. Removal of afferent input, by hindlimb denervation, failed to block the carrageenan-induced increase in iB-END levels. Subcutaneous perfusates were then collected from inflamed and control hindlimbs and applied to rat anterior pituitary cell cultures. Pituitary release of iB-END due to administration of perfusate from inflamed paws was significantly greater than iB-END release due to perfusate from saline-injected paws or to basal release. The releasing activity in the perfusates was blocked in calcium-free medium and was not due to a direct action of carrageenan, bradykinin, substance P or calcitonin gene-related peptide. The results indicate that inflamed tissue releases a CRF-like factor which stimulates iB-END release both in the denervated rat and cultured pituitary cells.|
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