Reference : Study by the sum-frequency generation spectroscopy of a model biosensor : The biocyti...
Scientific journals : Article
Physical, chemical, mathematical & earth Sciences : Physics
Study by the sum-frequency generation spectroscopy of a model biosensor : The biocytin-avidin complex*
Sartenaer, Y. [> >]
Dreesen, Laurent mailto [Université de Liège - ULg > Département de physique > Biophotonique >]
Humbert, Christophe [> >]
Mani, A. A. [> >]
Lemaire, J.-J. [> >]
Salmain, M. [> >]
Pradier, C.-M. [> >]
Thiry, P. A. [> >]
Peremans, A. [> >]
Physicalia Magazine
[en] Infrared-visible sum-frequency generation (SFG) spectroscopy is used to study the recognition of a protein (avidin) by a derivated vitamin (biocytin). No interaction is observed when the measurements are performed on metallic substrates in the normal co-propagative configuration. When the biocytin layer is deposited on a CaF2 substrate and the SFG measurements are performed in the total internal reflection configuration, drastic modifications in the C-H and N-H spectral ranges attest the molecular recognition of avidin by biocytin. The specificity of the recognition process is confirmed by replacing avidin by other proteins.
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