Reference : Probing Ligand–Protein Recognition with Sum-Frequency Generation Spectroscopy: The Av...
Scientific journals : Article
Physical, chemical, mathematical & earth Sciences : Physics
http://hdl.handle.net/2268/26723
Probing Ligand–Protein Recognition with Sum-Frequency Generation Spectroscopy: The Avidin–Biocytin Case
English
Dreesen, Laurent mailto [Université de Liège - ULg > Département de physique > Biophotonique >]
Sartenaer, Yannick [ > > ]
Humbert, Christophe [ > > ]
Mani, Alaa A. [ > > ]
Méthivier, Christophe [ > > ]
Pradier, Claire-Marie [ > > ]
Thiry, Paul [ > > ]
Peremans, André [ > > ]
2004
Chemphyschem : A European Journal of Chemical Physics and Physical Chemistry
Wiley-VCH Verlag Gmbh
5
1719-1725
Yes (verified by ORBi)
International
1439-4235
1439-7641
Weinheim
Germany
[en] Infrared/visible sum-frequency generation (SFG) spectroscopy is used to study the recognition of a protein (avidin) by a derived vitamin (biocytin) adsorbed on a calcium fluoride substrate. The specificity of the process is tested by replacing avidin with bovine serum albumin or presaturated avidin. The SFG spectroscopy shows drastic modifications in the CH and NH spectral ranges only upon exposure of the biocytin film to avidin. The comparison of the SFG data with Fourier transform infrared reflection absorption spectra (FT-IRRAS) in the same spectral ranges illustrates the advantages of nonlinear spectroscopy for studying and detecting recognition between biomolecules.
Researchers ; Professionals
http://hdl.handle.net/2268/26723

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