[en] Infrared/visible sum-frequency generation (SFG) spectroscopy is used to study the recognition of a protein (avidin) by a derived vitamin (biocytin) adsorbed on a calcium fluoride substrate. The specificity of the process is tested by replacing avidin with bovine serum albumin or presaturated avidin. The SFG spectroscopy shows drastic modifications in the CH and NH spectral ranges only upon exposure of the biocytin film to avidin. The comparison of the SFG data with Fourier transform infrared reflection absorption spectra (FT-IRRAS) in the same spectral ranges illustrates the advantages of nonlinear spectroscopy for studying and detecting recognition between biomolecules.
Disciplines :
Physics
Author, co-author :
Dreesen, Laurent ; Université de Liège - ULiège > Département de physique > Biophotonique
Sartenaer, Yannick
Humbert, Christophe
Mani, Alaa A.
Méthivier, Christophe
Pradier, Claire-Marie
Thiry, Paul
Peremans, André
Language :
English
Title :
Probing Ligand–Protein Recognition with Sum-Frequency Generation Spectroscopy: The Avidin–Biocytin Case
Publication date :
2004
Journal title :
Chemphyschem: A European Journal of Chemical Physics and Physical Chemistry
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