You are here:
| Reference : Active site-directed inhibition of E. coli DNA-dependent RNA polymerase by pyridoxal 5′-... |
| Scientific journals : Article | |||
| Life sciences : Biochemistry, biophysics & molecular biology | |||
| http://hdl.handle.net/2268/26715 | |||
| Active site-directed inhibition of E. coli DNA-dependent RNA polymerase by pyridoxal 5′-phosphate | |
| English | |
| Venegas, Alejandro [Universidad Católica de Chile > > > >] | |
Martial, Joseph  [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >] | |
| Valenzuela, Pablo [Universidad Católica de Chile > > > >] | |
| 19-Dec-1973 | |
| Biochemical and Biophysical Research Communications | |
| Academic Press | |
| 55 | |
| 4 | |
| 1053-1059 | |
| 0006-291X | |
| 1090-2104 | |
| San Diego | |
| CA | |
| [en] RNA polymerase ; E.coli ; pyridoxamine 5′-phosphate | |
| [en] DNA-dependent RNA polymerase from E. coli is rapidly inhibited by preincubation with pyridoxal 5′-phosphate. The enzyme is not inhibited by pyridoxamine 5′-phosphate or pyridoxine. Pyridoxal is about 10 times less effective than pyridoxal 5′-phosphate. Nucleoside triphosphates but not DNA, protect the enzyme from inhibition. Spectral data from the reaction mixture and NaBH4 reduction products indicate the formation of a Schiff base between the aldehyde group of pyridoxal 5′-phosphate and amino group of the enzyme. The results show that the inhibitor is reacting with a critical amino group presumably at the nucleoside phosphate binding site of the active center of the enzyme. | |
| http://hdl.handle.net/2268/26715 | |
| 10.1016/S0006-291X(73)80001-4 | |
| http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WBK-4G0VNMJ-GR&_user=532038&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000026659&_version=1&_urlVersion=0&_userid=532038&md5=041404f948e82600f1a2d6dc873c05ad |
There is no file associated with this reference.
All documents in ORBi are protected by a user license.
