Reference : Active site-directed inhibition of E. coli DNA-dependent RNA polymerase by pyridoxal ...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/26715
Active site-directed inhibition of E. coli DNA-dependent RNA polymerase by pyridoxal 5′-phosphate
English
Venegas, Alejandro [Universidad Católica de Chile > > > >]
Martial, Joseph mailto [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
Valenzuela, Pablo [Universidad Católica de Chile > > > >]
19-Dec-1973
Biochemical and Biophysical Research Communications
Academic Press
55
4
1053-1059
Yes (verified by ORBi)
0006-291X
1090-2104
San Diego
CA
[en] RNA polymerase ; E.coli ; pyridoxamine 5′-phosphate
[en] DNA-dependent RNA polymerase from E. coli is rapidly inhibited by preincubation with pyridoxal 5′-phosphate. The enzyme is not inhibited by pyridoxamine 5′-phosphate or pyridoxine. Pyridoxal is about 10 times less effective than pyridoxal 5′-phosphate. Nucleoside triphosphates but not DNA, protect the enzyme from inhibition. Spectral data from the reaction mixture and NaBH4 reduction products indicate the formation of a Schiff base between the aldehyde group of pyridoxal 5′-phosphate and amino group of the enzyme. The results show that the inhibitor is reacting with a critical amino group presumably at the nucleoside phosphate binding site of the active center of the enzyme.
http://hdl.handle.net/2268/26715
10.1016/S0006-291X(73)80001-4
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WBK-4G0VNMJ-GR&_user=532038&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000026659&_version=1&_urlVersion=0&_userid=532038&md5=041404f948e82600f1a2d6dc873c05ad

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