ORBi: Martial Joseph - Regulation of growth hormone messenger RNA by thyroid and glucocorticoid hormones

Reference : Regulation of growth hormone messenger RNA by thyroid and glucocorticoid hormones


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Biochemistry, biophysics & molecular biology
	To cite this reference:	http://hdl.handle.net/2268/26706

Title :	Regulation of growth hormone messenger RNA by thyroid and glucocorticoid hormones
Language :	English
Author, co-author :	Martial, Joseph [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
	Baxter, John D [> > > >]
	Goodman, Howard M [> > > >]
	Seeburg, Peter H [> > > >]
Publication date :	1977
Journal title :	Proceedings of the National Academy of Sciences of the United States of America
Publisher :	National Academy of Sciences
Volume :	74
Issue/season :	5
Pages :	1816-20
ISSN :	0027-8424
e-ISSN :	1091-6490
City :	Washington
Country :	DC
Keywords :	[en] Antigen-Antibody Reactions ; Cell Line ; Cell-Free System ; Dexamethasone/pharmacology ; Growth Hormone/biosynthesis/immunology ; Kinetics ; Nucleic Acid Hybridization ; Pituitary Gland/metabolism ; Poly A/metabolism ; Protein Precursors/biosynthesis/immunology ; RNA, Messenger/metabolism ; Triiodothyronine/pharmacology
Abstract :	[en] Thyroid and glucocorticoid hormones stimulate growth hormone synthesis in cultured rat pituitary cells (GC). We have compared changes in growth hormone production and mRNA in these cells. Triiodothyronine (10 nM) and dexamethasone (1 micron) stimulated increases in growth hormone production by 2.5- and 3.8-fold, respectively. There were corresponding increases in the capacity of RNA from hormone-treated cells to direct synthesis of pregrowth hormone in a wheat germ cell-free translation system, suggesting hormone-regulated increases in growth hormone mRNA. Hormone-induced changes in mRNA were also demonstrated by determining the kinetics of hybridization of a cDNA probe prepared from RNA enriched (about 70%) for growth hormone translational activity with RNA from control and hormone-treated cells. These results suggest that thyroid and glucocorticoid hormones can regulate growth hormone production by influencing the levels of its mRNA.
Permalink :	http://hdl.handle.net/2268/26706
Other URL :	http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=266704
Mentions required by the publisher for OA :	1977/05/01

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