No full text
Article (Scientific journals)
Decreased responsiveness of GH3 cells to the dopaminergic inhibition of prolactin
Faure, Nacia; Cronin, Michaël J; Martial, Joseph et al.
1980In Endocrinology, 107 (4), p. 1022-1026
Peer Reviewed verified by ORBi
 

Files


Full Text
No document available.

Send to



Details



Keywords :
Animals; Apomorphine/pharmacology; Bromocriptine/*pharmacology; Butaclamol/pharmacology; Cell Line; Clone Cells; Dopamine/*pharmacology; Dose-Response Relationship, Drug; Mice; Pituitary Neoplasms; Prolactin/*secretion
Abstract :
[en] The inhibitory regulation of PRL secretion by dopamine (DA) or the dopaminergic agonists bromergocryptine (CB-154) and apomorphine was studied in cultured GH3 cells, an established rat anterior pituitary cell line which produces both PRL and GH. The basal release of PRL from GH3 cells was unaffected when incubated for 6 h with DA concentrations as high as 10(-4) M. The inability of DA to suppress PRL secretion could not be explained by the catabolism of DA or the presence of unknown inhibitors (e.g. estradiol) in the fetal calf serum present in the incubation media. Apomorphine and CB-154 were only partially effective in suppressing PRL release at high concentrations of 10(-4) and 10(-5) M, respectively. Various concentrations of the dopaminergic antagonist d-butaclamol did not reverse the inhibitory action of 10(-5) M CB-154, while equal concentrations (10(-5) M) of both d- and l-butaclamol significantly suppressed PRL release. The greatly lowered responsiveness of GH3 cells to dopaminergic inhibition of PRL is suggestive of some impairment of DA receptors. This hypothesis is supported by radioligand binding studies in which high affinity dopaminergic binding sites are absent in the same cell line used in this study.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Faure, Nacia
Cronin, Michaël J
Martial, Joseph ;  Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Weiner, Richard I
Language :
English
Title :
Decreased responsiveness of GH3 cells to the dopaminergic inhibition of prolactin
Publication date :
1980
Journal title :
Endocrinology
ISSN :
0013-7227
eISSN :
1945-7170
Publisher :
Endocrine Society, Chevy Chase, United States - Maryland
Volume :
107
Issue :
4
Pages :
1022-1026
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 26 October 2009

Statistics


Number of views
53 (1 by ULiège)
Number of downloads
0 (0 by ULiège)

Scopus citations®
 
45
Scopus citations®
without self-citations
37
OpenCitations
 
37

Bibliography


Similar publications



Contact ORBi