|Reference : Inverse control of prolactin and growth hormone gene expression: effect of thyroliberin ...|
|Scientific journals : Article|
|Life sciences : Biochemistry, biophysics & molecular biology|
|Inverse control of prolactin and growth hormone gene expression: effect of thyroliberin on transcription and RNA stabilization|
|Laverriere, J. N. [> > > >]|
|Morin, A. [ > > ]|
|Tixier-Vidal, A. [> > > >]|
|Truong, A. T. [> > > >]|
|Gourdji, D. [> > > >]|
|Martial, Joseph [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]|
|Oxford University Press|
|Yes (verified by ORBi)|
|[en] Animals ; Gene Expression/drug effects ; Growth Hormone/*genetics ; Kinetics ; Pituitary Neoplasms/genetics/metabolism ; Prolactin/*genetics ; RNA Processing, Post-Transcriptional/drug effects ; RNA Stability/drug effects ; RNA, Messenger/genetics/metabolism ; RNA, Neoplasm/genetics/metabolism ; Rats ; Thyrotropin-Releasing Hormone/*pharmacology ; Transcription, Genetic/drug effects ; Tumor Cells, Cultured|
|[en] The hypothalamic tripeptide thyroliberin (TRH) regulates prolactin (PRL) and growth hormone (GH) synthesis inversely by modulating the levels of their specific mRNA. Changes in mRNA levels could involve both transcriptional and posttranscriptional events. To examine further these possibilities, we have investigated the effect of TRH on the biosynthesis and degradation of PRL and GH RNA in a rat pituitary tumor cell line. Newly synthesized PRL and GH RNA sequences were quantified in nuclear and cytoplasmic fractions by hybridization of 3H-labelled RNA to immobilized plasmid DNA containing either PRL or GH cDNA sequences. Steady-state levels of specific RNA were estimated by RNA blot hybridization. The results indicate that TRH increases in a rapid but transient manner the transcription of the PRL gene, and suggest that it does not alter the processing and the transport to the cytoplasm. In contrast, after a lag-time, TRH seems to induce a long-lasting inhibition on GH, as well as on overall gene transcription. Furthermore, we observed an effect of TRH on mRNA stability. TRH significantly increases the half-life of PRL mRNA. Our results also support the hypothesis that TRH decreases the half-life of GH mRNA. Such post-transcriptional action of TRH amplifies and prolongs the regulations exerted at the transcriptional level.|
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