|Reference : Molecular cloning of bovine viral diarrhea viral sequences|
|Scientific journals : Article|
|Life sciences : Biochemistry, biophysics & molecular biology|
|Molecular cloning of bovine viral diarrhea viral sequences|
|Renard, A. [ > > ]|
|Guiot, C. [> > > >]|
|Schmetz, D. [> > > >]|
|Dagenais, L. [> > > >]|
|Pastoret, Paul-Pierre [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Département des maladies infectieuses et parasitaires >]|
|Dina, D. [> > > >]|
|Martial, Joseph [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]|
|Mary Ann Liebert, Inc.|
|[en] Animals ; Base Sequence ; Cattle ; Cells, Cultured ; Chromosome Mapping ; Cloning, Molecular ; DNA/genetics ; Diarrhea Viruses, Bovine Viral/*genetics ; *Genes, Viral ; Pestivirus/*genetics ; Poly A/genetics ; RNA/genetics ; RNA, Messenger ; RNA, Viral/biosynthesis/genetics ; Virus Cultivation|
|[en] Bovine viral diarrhea virus (BVDV) genomic RNA was identified as a 12.5-kb single-stranded RNA molecule in both infected bovine embryonic kidney cells (BEK-1) and partially purified virions. BVD virion RNA was partially purified and used as a template for cDNA synthesis. BVDV-specific cDNA sequences were molecularly cloned and shown to hybridize to infected cell RNA but not to uninfected cell RNA or DNA. A single RNA species of 12.5 kb, representing the viral RNA genome, was detected in infected cells. A preliminary map of the BVDV specific cDNA clones was constructed and five major, nonoverlapping families were observed, accounting for approximately one-half of the viral genome.|
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