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| Reference : Molecular cloning of bovine viral diarrhea viral sequences |
| Scientific journals : Article | |||
| Life sciences : Biochemistry, biophysics & molecular biology | |||
| http://hdl.handle.net/2268/26665 | |||
| Molecular cloning of bovine viral diarrhea viral sequences | |
| English | |
| Renard, A. [ > > ] | |
| Guiot, C. [> > > >] | |
| Schmetz, D. [> > > >] | |
| Dagenais, L. [> > > >] | |
Pastoret, Paul-Pierre  [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Département des maladies infectieuses et parasitaires >] | |
| Dina, D. [> > > >] | |
| Martial, Joseph [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >] | |
| 1985 | |
| DNA | |
| Mary Ann Liebert, Inc. | |
| 4 | |
| 6 | |
| 429-38 | |
| 0198-0238 | |
| New York | |
| NY | |
| [en] Animals ; Base Sequence ; Cattle ; Cells, Cultured ; Chromosome Mapping ; Cloning, Molecular ; DNA/genetics ; Diarrhea Viruses, Bovine Viral/*genetics ; *Genes, Viral ; Pestivirus/*genetics ; Poly A/genetics ; RNA/genetics ; RNA, Messenger ; RNA, Viral/biosynthesis/genetics ; Virus Cultivation | |
| [en] Bovine viral diarrhea virus (BVDV) genomic RNA was identified as a 12.5-kb single-stranded RNA molecule in both infected bovine embryonic kidney cells (BEK-1) and partially purified virions. BVD virion RNA was partially purified and used as a template for cDNA synthesis. BVDV-specific cDNA sequences were molecularly cloned and shown to hybridize to infected cell RNA but not to uninfected cell RNA or DNA. A single RNA species of 12.5 kb, representing the viral RNA genome, was detected in infected cells. A preliminary map of the BVDV specific cDNA clones was constructed and five major, nonoverlapping families were observed, accounting for approximately one-half of the viral genome. | |
| http://hdl.handle.net/2268/26665 | |
| 1985/12/01 |
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