Reference : Sum-frequency generation spectroscopy of DNA monolayers
Scientific journals : Article
Physical, chemical, mathematical & earth Sciences : Physics
Sum-frequency generation spectroscopy of DNA monolayers
Sartenaer, Yannick [ > > ]
Tourillon, Gérard [ > > ]
Dreesen, Laurent mailto [Université de Liège - ULg > Département de physique > Biophotonique >]
Lis, Dan [ > > ]
Mani, Alaa A. [ > > ]
Thiry, Paul A. [ > > ]
Peremans, André [ > > ]
Biosensors & Bioelectronics
Yes (verified by ORBi)
United Kingdom
[en] The anchoring of thiolated single-strandedDNA(HS-ssDNA) monolayers onto platinum substrates was investigated by sum-frequency generation spectroscopy. Different buffer solutions were used for the preparation of the adlayers. Vibrational fingerprints in the 2700–3100 cm−1 spectral range showed the intercalation of Tris/EDTA (TE) buffer molecules within the HS-ssDNA self-assembled monolayer. Buffer contribution to SFG can be quenched either by using SFG inactive molecules like KH2PO4/K2HPO4/NaCl (PBS) or by repeated rinsing of the DNA layer with pure water. Comparing the SFG spectra of HS-ssDNA and mercaptohexanol (MCH), which had been self-assembled onto the same substrate, enabled us to infer ordering of the anchor arms and strong disordering of the DNA strands of HS-ssDNA monolayers self-assembled on platinum.
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