Reference : Sum-frequency generation spectroscopy of DNA monolayers
Scientific journals : Article
Physical, chemical, mathematical & earth Sciences : Physics
http://hdl.handle.net/2268/26608
Sum-frequency generation spectroscopy of DNA monolayers
English
Sartenaer, Yannick [ > > ]
Tourillon, Gérard [ > > ]
Dreesen, Laurent mailto [Université de Liège - ULg > Département de physique > Biophotonique >]
Lis, Dan [ > > ]
Mani, Alaa A. [ > > ]
Thiry, Paul A. [ > > ]
Peremans, André [ > > ]
2007
Biosensors & Bioelectronics
Elsevier
22
2179-2183
Yes (verified by ORBi)
International
0956-5663
Oxford
United Kingdom
[en] The anchoring of thiolated single-strandedDNA(HS-ssDNA) monolayers onto platinum substrates was investigated by sum-frequency generation spectroscopy. Different buffer solutions were used for the preparation of the adlayers. Vibrational fingerprints in the 2700–3100 cm−1 spectral range showed the intercalation of Tris/EDTA (TE) buffer molecules within the HS-ssDNA self-assembled monolayer. Buffer contribution to SFG can be quenched either by using SFG inactive molecules like KH2PO4/K2HPO4/NaCl (PBS) or by repeated rinsing of the DNA layer with pure water. Comparing the SFG spectra of HS-ssDNA and mercaptohexanol (MCH), which had been self-assembled onto the same substrate, enabled us to infer ordering of the anchor arms and strong disordering of the DNA strands of HS-ssDNA monolayers self-assembled on platinum.
Researchers ; Professionals
http://hdl.handle.net/2268/26608

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