ORBi: Dreesen Laurent - Adsorption Properties of the Penicillin Derivative DTPA on Gold Substrates

Reference : Adsorption Properties of the Penicillin Derivative DTPA on Gold Substrates


	Document type :	Scientific journals : Article
	Discipline(s) :	Physical, chemical, mathematical & earth Sciences : Physics
	To cite this reference:	http://hdl.handle.net/2268/26606

Title :	Adsorption Properties of the Penicillin Derivative DTPA on Gold Substrates
Language :	English
Author, co-author :	Dreesen, Laurent [Université de Liège - ULg > Département de physique > Biophotonique >]
	Silien, Christophe [> >]
	Volcke, Cédric [> >]
	Sartenaer, Yannick [> >]
	Thiry, Paul A. [> >]
	Peremans, André [> >]
	Grugier, Jérome [> >]
	Marchand-Brynaert, Jacqueline [> >]
	Brans, Alain [Université de Liège - ULg > > Centre d'ingénierie des protéines - GIGA-Management : Plate-forme production protéines >]
	Grubisic, Stana [Université de Liège - ULg > Services administratifs généraux > Protection et hygiène du travail (SUPHT) >]
	Joris, Bernard [Université de Liège - ULg > Département des sciences de la vie > Physiologie et génétique bactériennes - Centre d'ingénierie des protéines >]
Publication date :	2007
Journal title :	Chemphyschem : A European Journal of Chemical Physics and Physical Chemistry
Publisher :	Wiley-VCH Verlag Gmbh
Volume :	8
Issue/season :	7
Pages :	1071-1076
Audience :	International
ISSN :	1439-4235
e-ISSN :	1439-7641
City :	Weinheim
Country :	Germany
Keywords :	[en] antibiotics ; chemisorption ; immobilization ; monolayers ; synthetic methods
Abstract :	[en] Despite the large number of articles and patents dealing with penicillin and other b-lactam antibiotics, there have been no reports about the self-assembly of such substances as monolayers on gold surfaces. The main reason stems from the high reactivity of the b-lactam ring, which hinders the development of molecules possessing this entity together with a metal-anchoring function. Herein, we present the synthesis of a novel molecule, 6-[(R,S)-5-(1,2-dithiolan-3-yl)pentanoyl-amino]-penicillanic acid, which combines the b-lactam ring and a metal-anchoring group. Using spectroscopic tools, we demonstrate the chemisorption of this compound on gold as self-assembled monolayers without any alteration of the penicillin pharmacophore and document its reactivity towards a penicillin-binding protein, BlaR-CTD. Our work is a preliminary step towards the development of new biosensors and well-ordered protein arrays, both based on the high affinity of penicillin for penicillin-binding proteins.
Target :	Researchers ; Professionals
Permalink :	http://hdl.handle.net/2268/26606

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