ORBi: Rodriguez Christian - Development Of An Enzymatic Assay For The Determination Of Cellulose Bioavailability In Municipal Solid Waste

Reference : Development Of An Enzymatic Assay For The Determination Of Cellulose Bioavailability In ...


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Biochemistry, biophysics & molecular biology
	To cite this reference:	http://hdl.handle.net/2268/26409

Title :	Development Of An Enzymatic Assay For The Determination Of Cellulose Bioavailability In Municipal Solid Waste
Language :	English
Author, co-author :	Rodriguez, Christian [> >]
	Hiligsmann, Serge [Université de Liège > > Biochimie et microbiologie industrielles >]
	Ongena, Marc [Université de Liège - ULg > Chimie et bio-industries > Bio-industries >]
	Charlier, Robert [Université de Liège - ULg > Département Argenco : Secteur GEO3 > Géomécanique et géologie de l'ingénieur >]
	Thonart, Philippe [Université de Liège > > Gembloux Agro-Bio Tech >]
Publication date :	2005
Journal title :	Biodegradation
Volume :	16
Issue/season :	5
Pages :	415-422
Audience :	International
ISSN :	0923-9820
Keywords :	[en] biodegradation ; biochemical methane potential assay ; municipal solid waste ; cellulosic compounds ; enzymatic hydrolysis test
Abstract :	[en] As there is a constant need to assess the biodegradation potential of refuse disposed of in landfills, we have developed a method to evaluate the biodegradability of cellulosic compounds (cellulose and hemicellulose) in municipal solid waste. This test is based on the quantification of monosaccharids released after the hydrolysis of solid waste samples with an optimised enzyme preparation containing commercially available cellulases and hemicellulases. We show that the amounts of monosaccharids could be related to the biodegradability of the cellulosic material contained in the samples. This enzymatic cellulose degradation test was assayed on 26 samples originating from two Belgian landfills and collected at different depths. As results correlated well with those obtained with a classical biochemical methane potential assay, this new and rapid test is sufficiently reliable to evaluate cellulose bioavailability in waste samples.
Research units :	Centre Wallon de Biologie Industrielle
Funders :	Communauté française - Action de recherche concertée
Name of the research project :	Confinement de centres d'enfouissement technique à l'aide de barrières argileuses
Target :	Researchers ; Professionals ; Students ; General public
Permalink :	http://hdl.handle.net/2268/26409
DOI :	10.1007/s10532-004-3575-y

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