biodegradation; biochemical methane potential assay; municipal solid waste; cellulosic compounds; enzymatic hydrolysis test
Abstract :
[en] As there is a constant need to assess the biodegradation potential of refuse disposed of
in landfills, we have developed a method to evaluate the biodegradability of cellulosic
compounds (cellulose and hemicellulose) in municipal solid waste. This test is based on
the quantification of monosaccharids released after the hydrolysis of solid waste
samples with an optimised enzyme preparation containing commercially available
cellulases and hemicellulases. We show that the amounts of monosaccharids could be
related to the biodegradability of the cellulosic material contained in the samples. This
enzymatic cellulose degradation test was assayed on 26 samples originating from two
Belgian landfills and collected at different depths. As results correlated well with those
obtained with a classical biochemical methane potential assay, this new and rapid test is
sufficiently reliable to evaluate cellulose bioavailability in waste samples.
Research center :
Centre Wallon de Biologie Industrielle
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Rodriguez, Christian
Hiligsmann, Serge ; Université de Liège - ULiège > Biochimie et microbiologie industrielles
Ongena, Marc ; Université de Liège - ULiège > Chimie et bio-industries > Bio-industries
Charlier, Robert ; Université de Liège - ULiège > Département Argenco : Secteur GEO3 > Géomécanique et géologie de l'ingénieur
Thonart, Philippe ; Université de Liège - ULiège > Gembloux Agro-Bio Tech
Language :
English
Title :
Development Of An Enzymatic Assay For The Determination Of Cellulose Bioavailability In Municipal Solid Waste
Publication date :
2005
Journal title :
Biodegradation
ISSN :
0923-9820
eISSN :
1572-9729
Publisher :
Kluwer Academic Publishers, Netherlands
Volume :
16
Issue :
5
Pages :
415-422 415-422
Peer reviewed :
Peer Reviewed verified by ORBi
Name of the research project :
Confinement de centres d'enfouissement technique à l'aide de barrières argileuses
Funders :
Communauté française - Action de recherche concertée
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