Article (Scientific journals)
Characterization of the Insulin-Like Growth Factor Axis in the Human Thymus
Kecha, Ouafae; Martens, Henri; Franchimont, Nathalie et al.
1999In Journal of Neuroendocrinology, 11 (6), p. 435-40
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Abstract :
[en] The components of the insulin-like growth factor (IGF) axis have been investigated in the normal human thymus. Using ribonuclease protection assays (RPA), IGF-II transcripts were detected in the normal human thymus. By reverse transcriptase polymerase chain reaction (RT-PCR) analyses, promoters P3 and P4 were found to be active in the transcription of IGF2 gene within human thymic epithelial cells (TEC). No IGF-II mRNA could be detected in human lymphoid Jurkat T cells with 30 cycles of RT-PCR. By Northern blot analyses, IGFBP-2 to -6 (but not IGFBP-1) were found to be expressed in TEC with a predominance of IGFBP-4. Interestingly, Jurkat T cells only express IGFBP-2 but at high levels. The type 1 IGF receptor was detected in Jurkat T cells but not in human TEC. The identification of the components of the IGF axis within separate compartments of the human thymus adds further evidence for a role of this axis in the control of T-cell development. The precise influence of thymic IGF axis upon T-cell differentiation and immunological self-tolerance however needs to be further investigated.
Disciplines :
Immunology & infectious disease
Biochemistry, biophysics & molecular biology
Author, co-author :
Kecha, Ouafae
Martens, Henri ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Embryologie
Franchimont, Nathalie
Achour, Imane
Hazee-Hagelstein, Marie-Thérèse 
Charlet-Renard, Chantal
Geenen, Vincent ;  Université de Liège - ULiège > Centre d'immunologie
Winkler, Rose ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques
Language :
English
Title :
Characterization of the Insulin-Like Growth Factor Axis in the Human Thymus
Publication date :
June 1999
Journal title :
Journal of Neuroendocrinology
ISSN :
0953-8194
eISSN :
1365-2826
Publisher :
Wiley, Malden, United States - Massachusetts
Volume :
11
Issue :
6
Pages :
435-40
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 25 October 2009

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