Reference : Characterization of the Insulin-Like Growth Factor Axis in the Human Thymus
Scientific journals : Article
Human health sciences : Immunology & infectious disease
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/26404
Characterization of the Insulin-Like Growth Factor Axis in the Human Thymus
English
Kecha, Ouafae [> > > >]
Martens, Henri mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Embryologie >]
Franchimont, Nathalie [> > > >]
Achour, Imane [> > > >]
Hazee-Hagelstein, Marie-Thérèse [> > > >]
Charlet-Renard, Chantal [> > > >]
Geenen, Vincent mailto [Université de Liège - ULg > > Centre d'immunologie >]
Winkler, Rose mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
Jun-1999
Journal of Neuroendocrinology
11
6
435-40
Yes (verified by ORBi)
International
0953-8194
[en] The components of the insulin-like growth factor (IGF) axis have been investigated in the normal human thymus. Using ribonuclease protection assays (RPA), IGF-II transcripts were detected in the normal human thymus. By reverse transcriptase polymerase chain reaction (RT-PCR) analyses, promoters P3 and P4 were found to be active in the transcription of IGF2 gene within human thymic epithelial cells (TEC). No IGF-II mRNA could be detected in human lymphoid Jurkat T cells with 30 cycles of RT-PCR. By Northern blot analyses, IGFBP-2 to -6 (but not IGFBP-1) were found to be expressed in TEC with a predominance of IGFBP-4. Interestingly, Jurkat T cells only express IGFBP-2 but at high levels. The type 1 IGF receptor was detected in Jurkat T cells but not in human TEC. The identification of the components of the IGF axis within separate compartments of the human thymus adds further evidence for a role of this axis in the control of T-cell development. The precise influence of thymic IGF axis upon T-cell differentiation and immunological self-tolerance however needs to be further investigated.
http://hdl.handle.net/2268/26404

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