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Malignant catarrhal fever induced by alcelaphine herpesvirus 1 is associated with proliferation of CD8+ T cells supporting a latent infection
Dewals, Benjamin G; Boudry, Christel; Farnir, Frédéric et al.
20093rd European Veterinary Immunology Workshop
 

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Abstract :
[en] Alcelaphine herpesvirus 1 (AlHV 1), carried by wildebeest asymptomatically, causes malignant catarrhal fever (WD MCF) when cross species transmitted to a variety of susceptible species of the Artiodactyla order. Experimentally, WD-MCF can be induced in rabbits. The lesions observed are very similar to those described in natural host species. Here, we used the rabbit model and in vivo 5-Bromo-2’-Deoxyuridine (BrdU) incorporation to study WD-MCF pathogenesis. The results obtained can be summarized as follows. (i) AlHV-1 infection induces CD8+ T cell proliferation detectable as early as 15 days post-inoculation. (ii) While the viral load in peripheral blood mononuclear cells remains below the detection level during most of the incubation period, it increases drastically few days before death. At that time, at least 10% of CD8+ cells carry the viral genome; while CD11b+, IgM+ and CD4+ cells do not. (iii) RT-PCR analyses of mononuclear cells isolated from the spleen and the popliteal lymph node of infected rabbits revealed no expression of ORF25 and ORF9, low or no expression of ORF50, and high or no expression of ORF73. Based on these data, we propose a new model for the pathogenesis of WD-MCF. This model relies on proliferation of infected CD8+ cells supporting a predominantly latent infection.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Dewals, Benjamin G  ;  Université de Liège - ULiège > Immunologie et vaccinologie
Boudry, Christel 
Farnir, Frédéric  ;  Université de Liège - ULiège > Département de productions animales > Biostatistiques et bioinformatique appliquées aux sc. vétér.
Drion, Pierre ;  Université de Liège - ULiège > Services généraux (Faculté de médecine vétérinaire) > GIGA-R:Méth. expér.des anim. de labo et éth. en expér. anim. - GIGA-R : Services généraux de l'Université
Vanderplasschen, Alain ;  Université de Liège - ULiège > Immunologie et vaccinologie
Language :
English
Title :
Malignant catarrhal fever induced by alcelaphine herpesvirus 1 is associated with proliferation of CD8+ T cells supporting a latent infection
Publication date :
11 September 2009
Number of pages :
A0
Event name :
3rd European Veterinary Immunology Workshop
Event place :
Berlin, Germany
Event date :
10-13 september 2009
Audience :
International
Available on ORBi :
since 11 February 2010

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