Article (Scientific journals)
Expression of preprotachykinin-A and neuropeptide-Y messenger RNA in the thymus
Ericsson, Anders; Geenen, Vincent; Robert, François et al.
1990In Molecular Endocrinology, 4, p. 1211-1218
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Abstract :
[en] The preprotachykinin-A gene, the common gene of mRNAs encoding both substance-P (SP) and neurokinin- A (NKA), was shown to be expressed in Sprague-Dawley rat thymus by detection of specific mRNA in gel-blot analyses. In situ hybridization revealed dispersed PPT-A-labeled cells in sections from rat thymus, with a concentration of grains over a subpopulation of cells in the thymic medulla. Also, neuropeptide-Y mRNA-expressing cells were found in the rat thymus, primarily in the thymic medulla. Rat thymic extracts contained SP-like immunoreactivity (SP-LI), and the major part of the immunoreactivity coeluted with authentic SP and SP sulfoxide standards. SP-LI was also detected in human thymus, which contained between 0.09-0.88 ng SP-LI/ g wet wt. Evidence for translation of preprotachykinin- A mRNA in the rat thymus was obtained from the demonstration of NKA-LI in thymic cells with an epithelial-like cell morphology. Combined with previous observations on the immunoregulatory roles of tachykinin peptides and the existence of specific receptors on immunocompetent cells, the demonstration of intrathymic synthesis of NKA suggests a role for NKA-LI peptides in T-cell differentiation in the thymus.
Disciplines :
Immunology & infectious disease
Endocrinology, metabolism & nutrition
Author, co-author :
Ericsson, Anders
Geenen, Vincent ;  Université de Liège - ULiège > Centre d'immunologie - Embryologie
Robert, François
Legros, Jean-Jacques ;  Université de Liège - ULiège > Endocrinologie clinique
Vrindts-Gevaert, Yvonne
Franchimont, Paul
Brene, Stefan
Persson, Hakan
Language :
English
Title :
Expression of preprotachykinin-A and neuropeptide-Y messenger RNA in the thymus
Publication date :
1990
Journal title :
Molecular Endocrinology
ISSN :
0888-8809
eISSN :
1944-9917
Publisher :
Endocrine Society, Chevy Chase, United States - Maryland
Volume :
4
Pages :
1211-1218
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
Available on ORBi :
since 02 February 2011

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