immunoproteomics; multi-dimension; protein fractionation; PF2D
Abstract :
[en] We propose a multi-dimensional strategy, associating immunodetection to a protein fractionating two-dimensional liquid chromatography tool, for serological characterization of microbial antigens. The originality of such immunoproteomic approaches resides in their application in large-scale studies for rapid serotyping of micro-organisms, evaluation of immunomes and could be proposed in the development and monitoring of vaccines. (c) 2006 Elsevier B.V. All rights reserved.
Ruelle, Virginie ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine
Elmoualij, Benaïssa ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine
Zorzi, Danièle ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques
Pierard, Olivier ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine
Heinen, Ernst ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine
De Pauw, Edwin ; Université de Liège - ULiège > Chimie physique, spectrométrie de masse
Zorzi, Willy ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine
Language :
English
Title :
Advances in immunoproteomics for serological characterization of microbial antigens
Klade C.S. Proteomics approaches towards antigen discovery and vaccine development. Curr. Opin. Mol. Ther. 4 (2002) 216-223
Kyaw M.H., Clarke S., Edwards G.F., Jones I.G., and Campbell H. Serotypes/groups distribution and antimicrobial resistance of invasive pneumococcal isolates: implications for vaccine strategies. Epidemiol. Infect. 125 (2000) 561-572
Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 (1970) 680-685
Lubman D.M., Kachman M.T., Wang H., Gong S., Yan F., Hamler R.L., O'Neil K.A., Zhu K., Buchanan N.S., and Barder T.J. Two-dimensional liquid separations-mass mapping of proteins from human cancer cell lysates. J. Chromatogr., B, Anal. Technol. Biomed. Life Sci. 782 (2002) 183-196
Madoz-Gurpide J., Wang H., Misek D.E., Brichory F., and Hanash S.M. Protein based microarrays: a tool for probing the proteome of cancer cells and tissues. Proteomics 1 (2001) 1279-1287
Neverova I., and Van Eyk J.E. Role of chromatographic techniques in proteomic analysis. J. Chromatogr., B, Anal. Technol. Biomed. Life Sci. 815 (2005) 51-63
Steel L.F., Haab B.B., and Hanash S.M. Methods of comparative proteomic profiling for disease diagnostics. J. Chromatogr., B, Anal. Technol. Biomed. Life Sci. 815 (2005) 275-284
Vytvytska O., Nagy E., Bluggel M., Meyer H.E., Kurzbauer R., Huber L.A., and Klade C.S. Identification of vaccine candidate antigens of Staphylococcus aureus by serological proteome analysis. Proteomics 2 (2002) 580-590
Yan F., Sreekumar A., Laxman B., Chinnaiyan A.M., Lubman D.M., and Barder T.J. Protein microarrays using liquid phase fractionation of cell lysates. Proteomics 3 (2003) 1228-1235