Reference : Immunoquantitative real-time PCR for detection and quantification of Staphylococcus a...
Scientific journals : Article
Life sciences : Biotechnology
Life sciences : Microbiology
http://hdl.handle.net/2268/26232
Immunoquantitative real-time PCR for detection and quantification of Staphylococcus aureus enterotoxin B in foods
English
Rajkovic, A. [> > > >]
Elmoualij, Benaïssa mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie humaine >]
Uyttendaele, M. [> > > >]
Brolet, P. [> > > >]
Zorzi, Willy mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie humaine >]
Heinen, Ernst mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie humaine >]
Foubert, E. [> > > >]
Debevere, J. [> > > >]
Oct-2006
Applied and Environmental Microbiology
Amer Soc Microbiology
72
10
6593-6599
Yes (verified by ORBi)
International
0099-2240
Washington
[en] A real-time immunoquantitative PCR (iqPCR) method for detection of Staphylococcus aureus enterotoxin B (SEB) was developed and evaluated using both pure cultures and foods. The assay consisted of immunocapture of SEB and real-time PCR amplification of the DNA probe linked to the detection antibody. iqPCR was compared to an in-house enzyme-linked immunosorbent assay (ELISA) using the same couple of capture-detection antibodies and to commercial kits for detection of S. aureus enterotoxins (SE). The iqPCR was approximately 1,000 times more sensitive (< 10 pg ml(-1)) than the in-house ELISA and had a dynamic range of approximately 10 pg ml(-1) to approximately 30,000 pg ml(-1). iqPCR was not inhibited by any of the foods tested and was able to detect SEB present in these foods. No cross-reactivity with SE other than SEB was observed. Application of iqPCR for detection of SEB in cultures of S. aureus revealed the onset of SEB production after 4 It of incubation at 22, 37, and 42 degrees C, which was in the first half of the exponential growth phase. The total amounts of SEB produced by the two strains tested were larger at 42 degrees C than at 37 degrees C and were strain dependent.
http://hdl.handle.net/2268/26232

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