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| Reference : Novel plasma extraction procedure and development of a specific enzyme immunoassay of ox... |
| Scientific journals : Article | |||
| Human health sciences : Laboratory medicine & medical technology Human health sciences : Endocrinology, metabolism & nutrition | |||
| http://hdl.handle.net/2268/26069 | |||
| Novel plasma extraction procedure and development of a specific enzyme immunoassay of oxytocin: application to biological and clinical investigations of small-cell carcinoma of the lung | |
| English | |
Pequeux, Christel  [Université de Liège - ULg > Département des sciences cliniques > Labo de biologie des tumeurs et du développement >] | |
| Hendrick, Jean-Claude [ > > ] | |
| Hagelstein, Marie-Thérèse [Université de Liège - ULg > > Endocrinologie clinique >] | |
| Geenen, Vincent [Université de Liège - ULg > > Centre d'immunologie - Embryologie >] | |
| Legros, Jean-Jacques [Université de Liège - ULg > > Endocrinologie clinique >] | |
| 2001 | |
| Scandinavian Journal of Clinical & Laboratory Investigation | |
| Taylor & Francis Health Sciences | |
| 61 | |
| 407-415 | |
| International | |
| 0036-5513 | |
| 1502-7686 | |
| Oslo | |
| Norway | |
| [en] Paraneoplastic secretion of the lactation-inducing hormone oxytocin (OT) has been reported in about 30% of cases of small cell carcinoma of the lung (SCCL). In order to investigate the role of OT in the biology of SCCL tumours, a specific enzyme-immunoassay(EIA) for OT, which can be applied to both human plasma
and culturemedium, has been developed. OT EIA is performed on 96-well microtiter plates coated with a rabbit polyclonal antibody (Ab) anti-OT (O4). This antibody does not exhibit any significant cross-reactivity either with vasopressin (VP) or with vasotocin (VT). The immunological reaction involving Ab anti-OT is a competition between the tracer (biotinylatedOT) and syntheticOT (standard curve) or OT present in biological samples. In order to limit interference induced by plasma proteins, plasma samples are titrated by a one-step centrifugation on centricon YM-3 (cut-oOE 3000 Da). After plasma filtration, 90.7 ± 5.1 (SD) % (n = 22) immunoreactive ( IR) OT is recovered. The sensitivity of OT EIA is 1 pmol/L, while intra- and inter-assay coefficients of variation (CV) are around 3.41% and 2.84%, respectively. In healthy volunteers, plasma IR OT is 7.28 ± 4.49 (SD) pmol/L (n = 32) with no gender diOEerence. As shown by the data both from plasma of SCCL patients and from supernatants and cell contents of SCCL cell lines, this EIA procedure offers a novel, reproducible, specific and sensitive method for the measurement of IR OT. | |
| http://hdl.handle.net/2268/26069 |
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