Reference : Oxytocin synthesis and oxytocin receptor expression by cell lines of human small cell...
Scientific journals : Article
Human health sciences : Oncology
http://hdl.handle.net/2268/25860
Oxytocin synthesis and oxytocin receptor expression by cell lines of human small cell carcinoma of the lung stimulate tumor growth through autocrine/paracrine signaling
English
Pequeux, Christel mailto [Université de Liège - ULg > Département des sciences cliniques > Labo de biologie des tumeurs et du développement >]
Breton, Christophe [> > > >]
Hendrick, Jean-Claude [> > > >]
Hagelstein, Marie-Thérèse mailto [Université de Liège - ULg > > Endocrinologie clinique >]
Martens, Henri mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Embryologie >]
Winkler, Rose mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
Geenen, Vincent mailto [Université de Liège - ULg > > Centre d'immunologie >]
Legros, Jean-Jacques mailto [Université de Liège - ULg > Département des sciences cliniques > Département des sciences cliniques >]
15-Aug-2002
Cancer Research
American Association for Cancer Research (AACR)
62
16
4623-4629
Yes (verified by ORBi)
International
0008-5472
1538-7445
Birmingham
MD
[en] The objective of the present work was to investigate the existence of an oxytocin (OT)-mediated autocrine/paracrine signaling upon small cell carcinoma of the lung (SCCL) cell growth. In that view, OT receptor (OTR) expression, concomitant with OT synthesis and secretion, was evidenced on three different SCCL cell lines (DMS79, H146, and H345) and related to the vasopressin (VP) system. Specific OT, VP, OTR, Via VP receptor (V1aR), and V1b/V3 VP receptor (V1bR/V3R) transcripts were identified by reverse transcription-.PCR in all cell lines studied. Binding of I-125-(d(CH2)(5)(1),Tyr(Me)(2), Thr(4), Orn(3),Tyr(9)-NH2)-vasotocin (OVTA) was observed on all SCCL cell lines, with a K-d (dissociation constant) ranging from 0.025-0.089 nm, depending; on the cell line and the analytical method. Selectivity of I-125-OVTA binding was confirmed by displacement curves obtained with various OTR and VP receptor agonists and antagonists (OT, OVTA, L-371,257, VP, F180). Immunocytochemistry identified cellular OT and VP, and peptide secretion was measured in supernatants of SCCL cultures. [H-3]Thymidine incorporations, applied on H345 cells, demonstrated a dose-dependent mitogenic effect of exogenous OT (1 and 100 nM) that was abolished by the OTR antagonist OVTA. A decrease of proliferation was also observed with OVTA alone, showing a functional mitogenic effect of tumor-derived OT. Taken together, these observations demonstrate the existence of a functional OT-mediated autocrine/paracrine signaling actively implicated in growth and development of SCCL tumors. Furthermore, these findings point to the potential of OT antagonists for development as therapeutic agents for the treatment of SCCL.
http://hdl.handle.net/2268/25860
also: http://hdl.handle.net/2268/26423

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