Article (Scientific journals)
Involvement of Insulin-Like Growth Factors in Early T Cell Development: A Study Using Fetal Thymic Organ Cultures
Kecha, O.; Brilot, F.; Martens, Henri et al.
2000In Endocrinology, 141 (3), p. 1209-17
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Abstract :
[en] The expression of insulin-like growth factor (IGF) and IGF receptor genes was investigated by RT-PCR during ontogeny of the murine thymus. IGF-1, IGF-1R, M6P/IGF-2R genes are expressed in the thymus both in fetal and postnatal life, whereas IGF-2 messenger RNAs (mRNAs) decline after birth but are still detectable on the seventh week. By in situ hybridization, IGF-2 transcripts were located in the outer cortex and medulla of the postnatal thymus, and on the whole surface ofthe epithelial-like network in the fetal thymus. The effects of anti-IGFs and IGF-receptors neutralizing Abs on the generation of pre-T cell subpopulations were then investigated using fetal thymic organ cultures (FTOC). FTOC treatment with an anti-IGF-2 mAb, an anti-IGF-1R mAb, or an anti-M6P/IGF-2R polyclonal Ab induced a blockade of T cell differentiation at the CD4-CD8- stage, as shown by a significant increase in the percentage of CD4-CD8- cells and a decrease in the percentage of CD4+CD8+ cells. Moreover, anti-IGF-2 Ab treatment induced an increase in CD8+ cells suggesting that thymic IGF-2 might have a role in determining differentiation into the CD4 or CD8 lineage. Anti-IGF-1 Ab treatment decreased the proportion in CD4-CD8- cells and increased the frequency in CD4+CD8+. FTOC treatment with anti-(pro)insulin did not exert any significant effect on T cell development. These data indicate that the intrathymic IGF-mediated signaling plays an active role in the early steps of T cell differentiation during fetal development.
Disciplines :
Endocrinology, metabolism & nutrition
Immunology & infectious disease
Author, co-author :
Kecha, O.
Brilot, F.
Martens, Henri ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Embryologie
Franchimont, N.
Renard, Cécile ;  Centre Hospitalier Universitaire de Liège - CHU > Rééducation de la parole
Greimers, Roland ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques
Defresne, Marie-Paule ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie - Cytologie
Winkler, Rose ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques
Geenen, Vincent ;  Université de Liège - ULiège > Centre d'immunologie
Language :
English
Title :
Involvement of Insulin-Like Growth Factors in Early T Cell Development: A Study Using Fetal Thymic Organ Cultures
Publication date :
March 2000
Journal title :
Endocrinology
ISSN :
0013-7227
eISSN :
1945-7170
Publisher :
The Endocrine Society, United States - Maryland
Volume :
141
Issue :
3
Pages :
1209-17
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 18 October 2009

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