Reference : Involvement of Insulin-Like Growth Factors in Early T Cell Development: A Study Using Fe...
Scientific journals : Article
Human health sciences : Endocrinology, metabolism & nutrition
Human health sciences : Immunology & infectious disease
http://hdl.handle.net/2268/25855
Involvement of Insulin-Like Growth Factors in Early T Cell Development: A Study Using Fetal Thymic Organ Cultures
English
Kecha, O. [> > > >]
Brilot, F. [> > > >]
Martens, Henri mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Embryologie >]
Franchimont, N. [> > > >]
Renard, Cécile [Centre Hospitalier Universitaire de Liège - CHU > > Rééducation de la parole >]
Greimers, Roland mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
Defresne, Marie-Paule mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie - Cytologie >]
Winkler, Rose mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
Geenen, Vincent mailto [Université de Liège - ULg > > Centre d'immunologie >]
Mar-2000
Endocrinology
141
3
1209-17
Yes (verified by ORBi)
International
0013-7227
[en] The expression of insulin-like growth factor (IGF) and IGF receptor genes was investigated by RT-PCR during ontogeny of the murine thymus. IGF-1, IGF-1R, M6P/IGF-2R genes are expressed in the thymus both in fetal and postnatal life, whereas IGF-2 messenger RNAs (mRNAs) decline after birth but are still detectable on the seventh week. By in situ hybridization, IGF-2 transcripts were located in the outer cortex and medulla of the postnatal thymus, and on the whole surface ofthe epithelial-like network in the fetal thymus. The effects of anti-IGFs and IGF-receptors neutralizing Abs on the generation of pre-T cell subpopulations were then investigated using fetal thymic organ cultures (FTOC). FTOC treatment with an anti-IGF-2 mAb, an anti-IGF-1R mAb, or an anti-M6P/IGF-2R polyclonal Ab induced a blockade of T cell differentiation at the CD4-CD8- stage, as shown by a significant increase in the percentage of CD4-CD8- cells and a decrease in the percentage of CD4+CD8+ cells. Moreover, anti-IGF-2 Ab treatment induced an increase in CD8+ cells suggesting that thymic IGF-2 might have a role in determining differentiation into the CD4 or CD8 lineage. Anti-IGF-1 Ab treatment decreased the proportion in CD4-CD8- cells and increased the frequency in CD4+CD8+. FTOC treatment with anti-(pro)insulin did not exert any significant effect on T cell development. These data indicate that the intrathymic IGF-mediated signaling plays an active role in the early steps of T cell differentiation during fetal development.
http://hdl.handle.net/2268/25855

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