[en] OBJECTIVE: To isolate new cDNAs corresponding to genes whose expression is increased during castration-induced rat prostate apoptosis. MATERIALS AND METHODS: Differential display of mRNAs from 3-day castrated and normal rat ventral prostates was used to identify differentially expressed clones. Northern blots were hybridized to confirm the positive regulation of the candidates and to follow the change in their expression in the involuting rat prostate, and in thymocytes of dexamethazone-treated rats. RESULTS: Five cDNAs were cloned: one encoding ribosomal protein L7, one coding for the insulin-like growth factor binding protein-3 (IGFBP-3), and three whose products are unknown. After castration, all five genes had expression kinetics that closely paralleled the proportion of prostatic epithelial cells undergoing apoptosis. The gene encoding L7 and two of the unknown genes were also upregulated in glucocorticoid-induced programmed death in thymocytes. In addition to the IGFBP-3 gene, those coding for proteins IGFBP-4, -5 and -6 were also overexpressed in the involuting prostate of androgen-deprived rats. CONCLUSION: Five new genes were identified that are up-regulated during castration-induced rat prostate apoptosis, three of which are potentially involved in the common intracellular pathway leading to programmed cell death.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Bruyninx, M.
Ammar, H.
Reiter, E.
Cornet, Anne ; Université de Liège - ULiège > Département de morphologie et pathologie > Pathologie spéciale et autopsies
Closset, Jean ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques
Language :
English
Title :
Genes upregulated during castration-induced rat prostatic apoptosis: cloning and characterization of new cDNAs.
Isaacs JT. Antagonistic effect of androgen on prostatic cell death. Prostate 1984; 5: 545-57
Kyprianou N, Isaacs JT. Activation of programmed cell death in the rat ventral prostate after castration. Endocrinology 1988; 122: 552-62
English HF, Kyprianou N, Isaacs JT. Relationship between DNA fragmentation and apoptosis in the programmed cell death in the rat prostate following castration. Prostate 1989; 15: 233-51
English HF, Drago JR, Santen RJ. Cellular response to androgen depletion and repletion in the rat ventral prostate: autoradiography and morphometric analysis. Prostate 1985; 7: 41-51
English HF, Santen RJ, Isaacs JT. Response of glandular vs. basal rat ventral prostatic epithelial cells to androgen withdrawal and replacement. Prostate 1987; 11: 229-42
Rouleau M, Leger JG, Tenniswood M. Ductal heterogeneity of cytokeratins, gene expression and cell death in the rat ventral prostate. Mol Endocrinol 1990; 4: 2003-13
Suzuki A, Mutsuzawa A, Iguchi T. Down regulation of bcl-2 is the first step in fas-mediated apoptosis of male reproductive tract. Oncogene 1996; 13: 31-7
de la Taille A, Chen MW, Shabsigh A, Kiss A, Buttyan R. Fas antigen/CD-95 upregulation and activation during castration-induced regression of the rat ventral prostate gland. Prostate 1999; 40: 89-96
Kyprianou N, Isaacs JT. Expression of transforming growth factor-β in the rat ventral prostate during castration induced programmed cell death. Mol Endocrinol 1989; 3: 1515-22
Kyprianou N, Isaacs JT. Identification of a cellular receptor for transforming growth factor beta in rat ventral prostate and its negative regulation by androgens. Endocrinology 1988; 123: 2124-31
Kim IY, Ahn HJ, Zelner DJ, Park L, Sensibar JA, Lee C. Expression and localisation of transforming growth factor-β receptor type I and type II in the rat ventral prostate during regression. Mol Endocrinol 1996; 10: 107-15
Brodin G, ten Dijke P, Funa K, Heldin CH, Landstrom M. Increased smad expression and activation are associated with apoptosis in normal and malignant prostate after castration. Cancer Res 1999; 59: 2731-8
Rajah R, Valentinis B, Cohen P. Insulin-like growth factor (IGF)-binding protein-3 induces apoptosis and mediates the effects of transforming growth factor-β1 on programmed cell death trough a p53- and IGF-independent mechanism. J Biol Chem 1997; 272: 12181-8
Guo Y, Kyprianou N. Restoration of transforming growth factor beta signaling pathway in human prostate cancer cells suppresses tumorigenicity via induction of caspase-1-mediated apoptosis. Cancer Res 1999; 59: 1366-71
Bowen C, Voeller HJ, Kikly K, Gelmann EP. Synthesis of procaspases-3 and -7 during apoptosis in prostate cancer cells. Cell Death Differ 1999; 6: 394-401
Wang JD, Takahara S, Nonomura N et al. Early induction of apoptosis in androgen-independent prostate cancer cell line by FTY720 requires caspase-3 activation. Prostate 1999; 40: 50-5
Denmeade SR, Lin XS, Tombal B, Isaacs JT. Inhibition of caspase activity does not prevent the signaling phase of apoptosis in prostate cancer cells. Prostate 1999; 39: 269-79
Montpetit ML, Lawless KR, Tenniswood M. Androgen-repressed messages in the rat ventral prostate. Prostate 1986; 8: 25-36
Chang C, Saltzman AG, Sorensen NS, Hiipakka RA, Liao S. Identification of glulathione S-transferase Yb1 mRNA as the androgen repressed mRNA by cDNA cloning and sequence analysis. J Biol Chem 1987; 262: 11901-3
Guenette RS, Mooibroek M, Wong K, Wong P, Tenniswood M. Cathepsin B, a cystein protease implicated in metastatic progression, is also expressed during regression of the rat prostate and mammary glands. Eur J Biochem 1994; 226: 311-21
Powell WC, Domann FE, Mitchen JM, Matrisian LM, Nagle RB, Bowden GT. Matrilysin expression in the involuting rat ventral prostate. Prostate 1996; 29: 159-68
Rauch F, Polzar B, Stephan H, Zanotti S, Paddenberg R, Mannherz HG. Androgen ablation leads to an upregulation and intranuclear accumulation of deoxyribonuclease I in rat prostate epithelial cells paralleling their apoptotic elimination. J Cell Biol 1997; 137: 909-23
Liang P, Pardee AB. Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction. Science 1992; 257: 967-71
Chomczinski P, Sacchi N. Single step method of RNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction. Anal Biochem 1987; 162: 156-9
Bishop JO. The gene numbers game. Cell 1974; 2: 81-5
Nagase T, Ishikawa K, Nakajima D et al. Prediction of the coding sequences of unidentified human genes. VII. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro. DNA Res 1997; 4: 141-50
Osborne BA. Induction of genes during apoptosis: examples from the immune system. Semin Cancer Biol 1995; 6: 27-33
Nishi N, Oya H, Matsumoto K, Nakamura T, Miyanaka H, Wada F. Changes in gene expression of growth factors and their receptors during castration-induced involution and androgen-induced regrowth of rat prostates. Prostate 1996; 28: 139-52
Neumann F, Hemmerich P, von Mikecz A, Peter HH, Krawinkel U. Human ribosomal protein L7 inhibits cell-free translation in reticulocyte lysates and affects the expression of nuclear proteins upon stable transaction into Jurkat T-lymphoma cells. Nucl Acids Res 1995; 23: 195-202
Neumann F, Krawinkel U. Constitutive expression of human ribosomal protein L7 arrests the cell cycle in G1 and induces apoptosis in Jurkat T-lymphoma cells. Exp Cell Res 1997; 230: 252-61
Bruyninx M, Hennuy B, Cornet A et al. A novel gene overexpressed in the prostate of castrated rats: hormonal regulation, relationship to apoptosis and to acquired prostatic cell androgen-independence. Endocrinology 1999; 140: 4789-99
Gubbay J, Doyle JP, Skinner M, Heintz N. Changing patterns of gene expression identify multiple step during regression of rat prostate in vivo. Endocrinology 1998; 139: 2935-43
Cohen P, Peehl DM, Graves HCB, Rosenfeld GR. Biological effects of prostate specific antigen as an insulin-like growth factor binding protein-3 protease. J Endocrinol 1994; 142: 407-15
Leal SM, Liu Q, Huang SS, Huang JS. The type V transforming growth factor β receptor is the putative insulin-like growth factor-binding protein 3 receptor. J Biol Chem 1997; 272: 20572-6
Monget P, Monniaux D, Pisselet C, Durand P. Changes in insulin-like growth fattor-I (IGF-I), IGF-II, and their binding proteins during growth and atresia of ovine ovarian follicles. Endocrinology 1993; 132: 1438-46
Phillips ID, Becks GP, Logan A, Wang JF, Smith C, Hill DJ. Altered expression of insulin-like growth factor-I (IGF-I) and IGF binding proteins during rat thyroid hyperplasia and involution. Growth Factors 1994; 10: 207-22
Nickerson T, Pollak M, Huynh H. Castration-induced apoptosis in the rat ventral prostate is associated with increased expression of genes encoding insulin-like growth factor binding proteins 2, 3, 4 and 5. Endocrinology 1998: 139: 807-10
Nickerson T, Huynh H. Vitamin D analogue EB1089-induced prostate regression is associated with increased gene expression of insulin-like growth factor binding proteins. J Endocrinol 1999; 160: 223-9
Thomas LN, Cohen P, Douglas RC, Lazier C, Rittmaster RS. Insulin-like growth factor binding protein 5 is associated with involution of the ventral prostate in castrated and finasteride-treated rats. Prostate 1998; 35: 273-8
Bach LA. Insulin-like growth factor binding protein-6: the 'forgotten' binding protein? Horm Metab Res 1999; 31: 226-34
Delhanty PJD, Han VKM. An RCD to RGE mutation in the putative membrane binding domain of insulin-like growth factor binding protein-2 inhibits its potentiation of IGF-II induced thymidine uptake by SPC cells. Proceedings of the 75th Annual Meeting of the Endocrine Society 1993: 56
Jones JI, Gockerman A, Busby WH, Wright G, Clemmons DR. Insulin-like growth factor binding protein-2 stimulates cell migration and binds to the α5βN1 integrin by means of its Arg-Gly-Asp sequence. Proc Natl Acad Sci USA 1993; 90: 10553-7
